(4-Nitrophenyl)-thymidin-3'-carbonat | 10270-36-7

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: (4-Nitrophenyl)-thymidin-3'-carbonat
• 英文别名: O^3'-(4-nitro-phenoxycarbonyl)-thymidine；[(2R,3S,5R)-2-(hydroxymethyl)-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-3-yl] (4-nitrophenyl) carbonate
• CAS: 10270-36-7
• 化学式: C₁₇H₁₇N₃O₉
• 分子量: 407.337
• InChiKey: QCXMENRVHFLPEM-BFHYXJOUSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.1
• 重原子数：
  29
• 可旋转键数：
  6
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.35
• 拓扑面积：
  160
• 氢给体数：
  2
• 氢受体数：
  9

文献信息

• Method for covalently attaching nucleosides and/or nucleotides on surfaces and method for determining coupling yields in the synthesis of nucleotides
  申请人：Stengele Klaus-Peter

  公开号：US20060154256A1

  公开（公告）日：2006-07-13

  The present invention relates to a method for covalently attaching nucleosides and/or nucleotides on surfaces having reactive functional groups, where in a first step, the reactive functional groups are made to react with suitable derivatized nucleosides and/or nucleotides, and in a second step, they are converted with a protecting group reagent, so that a reaction product of the consecutive reaction interacts with electromagnetic radiation such that it can be quantitatively determined. The invention also relates to a method for determining the repetitive coupling yields in the synthesis of nucleotides where the free 3′ or 5′ hydroxy group of a selected nucleoside and/or nucleotide is converted with a compound of formula (I) where L is a common suitable leaving group, the motif O—PX represents a phosphor amidite, a H-phosphonate a phosphonic acid ester, a phosphotriester, Y═O or S, N is a nucleoside or a nucleotide derivative which subsequently reacts further with a protecting group reagent and the elimination of the leaving group (L), which is subsequently further eliminated. The quantity of the leaving group (L) eliminated in step b) is quantitatively determined in the form of its anion (L − ) by means of optical spectroscopy.

  本发明涉及一种在具有反应性功能团的表面上共价连接核苷和/或核苷酸的方法。其中，在第一步中，反应性功能团与适当衍生的核苷和/或核苷酸发生反应，在第二步中，它们与保护基试剂转化，使得连续反应的反应产物与电磁辐射相互作用，从而可以定量测定。该发明还涉及一种确定核苷酸合成中重复偶联收率的方法，其中选择的核苷和/或核苷酸的自由3'或5'羟基转化为化合物(I)的形式，其中L是一种常见的适当离去基，O-PX基序列代表磷酰胺、H-膦酸酯、磷酸酯、磷酸三酯，Y=O或S，N是一个核苷或核苷酸衍生物，随后进一步与保护基试剂反应，并消除离去基(L)，随后进一步消除。步骤b)中消除的离去基(L)的数量以其阴离子(L-)的形式通过光谱学定量测定。
• Multimer polynucleotide synthesis
  申请人：——

  公开号：US20040203036A1

  公开（公告）日：2004-10-14

  The present invention relates to a process for the preparation of polynucleotides, whereby under suitable usual conditions the free 5′-hydroxy group, whose terminal 3′-hydroxy group contains a usual protecting group, is reacted with a hydroxy group, derivatized in a previous reaction step to a phosphite amidoester, phosphpotriester or phosphonic acid ester, whereby said hydroxy group is a 3′-hydroxy function of a free or solid phase bound polynucleotide, or a solid phase bound hydroxy function. Further the present invention relates to a kit for performing a process according to the invention, which contains at least one or more selected oligonucleotides, having a free 5′-hydroxy group and a protected 3′-hydroxy group. Further on, the present invention relates to new oligonucleotides and their use as building blocks for the synthesis of polynucleotides in the process according to the invention. Furthermore the present invention relates to the use of the process according to the invention or the use of the kits for the preparation of poly/oligonucleotides resp. polynucleotide libraries or nucleic acid chips.

  本发明涉及一种制备多核苷酸的工艺，在适当的通常条件下，游离的5′-羟基（其末端3′-羟基含有通常的保护基团）与羟基反应、在前一反应步骤中衍生为亚磷酸酰胺酯、亚磷酸三酯或膦酸酯，其中所述羟基是游离的或固相结合的多核苷酸的 3′-羟基官能团，或固相结合的羟基官能团。 本发明还涉及一种用于实施本发明工艺的试剂盒，该试剂盒含有至少一种或多种选定的寡核苷酸，这些寡核苷酸具有游离的 5′-羟基和受保护的 3′-羟基。此外，本发明还涉及新的寡核苷酸及其在根据本发明的工艺中作为合成多核苷酸的构件的用途。 此外，本发明还涉及根据本发明的工艺或试剂盒在制备多核苷酸/寡核苷酸以及多核苷酸文库或核酸芯片中的用途。
• MULTIMER POLYNUCLEOTIDE SYNTHESIS
  申请人：Chemogenix GmbH

  公开号：EP1409505A1

  公开（公告）日：2004-04-21
• METHOD FOR COVALENTLY ATTACHING NUCLEOSIDES AND/OR NUCLEOTIDES ON SURFACES AND METHOD FOR DETERMINING COUPLING YIELDS IN THE SYNTHESIS OF NUCLEOTIDES
  申请人：Chemogenix GmbH

  公开号：EP1438322A2

  公开（公告）日：2004-07-21
• [EN] MULTIMER POLYNUCLEOTIDE SYNTHESIS<br/>[FR] SYNTHESE DE POLYNUCLEOTIDES MULTIMERES
  申请人：CHEMOGENIX GMBH

  公开号：WO2003006476A1

  公开（公告）日：2003-01-23

  The present invention relates to a process for the preparation of polynucleotides, whereby under suitable usual conditions the free 5'-hydroxy group, whose terminal 3'-hydroxy group contains a usual protecting group, is reacted with a hydroxy group, derivatized in a previous reaction step to a phosphite amidoester, phosphpotriester or phosphonic acid ester, whereby said hydroxy group is a 3'-hydroxy function of a free or solid phase bound polynucleotide, or a solid phase bound hydroxy function. Further the present invention relates to a kit for performing a process according to the invention, which contains at least one or more selected oligonucleotides, having a free 5'-hydroxy group and a protected 3'-hydroxy group. Further on, the present invention relates to new oligonucleotides and their use as building blocks for the synthesis of polynucleotides in the process according to the invention. Furthermore the present invention relates to the use of the process according to the invention or the use of the kits for the preparation of poly/oligonucleotides resp. polynucleotide libraries or nucleic acid chips.