fluorescein bis(3-nitrobenzoate) | 1262883-54-4

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: fluorescein bis(3-nitrobenzoate)
• 英文别名: 3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diacyl bis(3-nitrobenzoate)
• CAS: 1262883-54-4
• 化学式: C₃₄H₁₈N₂O₁₁
• 分子量: 630.524
• InChiKey: LCTSCLALVCRCPU-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  6.51
• 重原子数：
  47.0
• 可旋转键数：
  6.0
• 环数：
  7.0
• sp3杂化的碳原子比例：
  0.03
• 拓扑面积：
  174.41
• 氢给体数：
  0.0
• 氢受体数：
  11.0

反应信息

• 作为产物：
  描述：

  间硝基苯甲酸 、 荧光素 在 四磷十氧化物 作用下， 反应 4.0h， 以95%的产率得到fluorescein bis(3-nitrobenzoate)
  参考文献：
  名称：

  Synthesis of fluorescein aromatic esters in the presence of P2O5/SiO2 as catalyst and their hydrolysis studies in the presence of lipase

  摘要：

  A series of fluorescein aryl esters were synthesized by the esterification of fluorescein with carboxylic acids in the presence of P2O5/SiO2 and their hydrolytic properties were investigated. The rate of hydrolysis in the presence or absence of lipase, due to the increase of fluorescein concentration, was measured by monitoring of fluorescence of the solution and correlated with enzyme activity. In addition, the substitute effect on the aromatic ring of fluorescein esters was studied. In contrast to fluorescein diacetate or dibutyrate, fluorescein dibenzoate and fluorescein bis(4-methylbenzoate) were found to be better substrates for the fluorometric assay of lipase with the higher rate of hydrolysis and better K-m value, respectively. As little as 9.3 ng mL(-1) of lipase can be detected with fluorescein bis(4-methylbenzoate). (C) 2010 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.dyepig.2010.09.013

文献信息

• 一种肼可视化荧光探针分子及其制备方法
  申请人：中国科学院新疆理化技术研究所

  公开号：CN113321662A

  公开（公告）日：2021-08-31

  本发明提供一种肼可视化荧光探针分子及其制备方法，采用将化合物4‑二甲氨基吡啶、1‑(3‑二甲氨基丙基)‑3‑乙基碳二亚胺盐酸盐、3‑R基苯甲酸和荧光素混合，室温条件下反应完全后，加入二氯甲烷溶剂，得到粗产物；将粗产物经正向硅胶色谱柱纯化，得到白色固体检测肼的荧光探针分子3‑氧代‑3H‑螺[异苯并呋喃‑1,9'‑氧杂蒽]‑3',6'‑二酰双(3‑R基苯甲酸酯)。当肼与荧光探针分子酯键反应，去除识别部分而将荧光发射中心分离，生成具有强列绿色荧光发射的荧光素，通过荧光颜色信号变化实现肼的定性检测。本发明制备过程简单，成本低，兼具优异的检测选择性和灵敏性，能够用于快速定性检测肼，具备广阔的应用前景。