硫酸耐尔蓝 | 3625-57-8

• 物质功能分类: 生物化工 - 生化试剂 - 生物染料
• 分子结构分类: 有机化合物 - 有机杂环化合物
• 中文名称: 硫酸耐尔蓝
• 中文别名: 耐尔兰；尼尔兰
• 英文名称: nile blue A
• 英文别名: 5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium sulfate；5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium sulfate；Nile blue sulfate；Nile blue；5-amino-9-diethylamino-benzo[a]phenoxazinylium; sulfate
• CAS: 3625-57-8
• 化学式: 2C₂₀H₂₀N₃O*O₄S
• 分子量: 732.86
• InChiKey: MMYKGGIXVBGGSZ-UHFFFAOYSA-L

物化性质

• 熔点：
  >300 °C (dec.)(lit.)
• 溶解度：
  易溶于水、乙醇
• 最大波长(λmax)：
  633 nm

计算性质

• 辛醇/水分配系数(LogP)：
  3.51
• 重原子数：
  29.0
• 可旋转键数：
  3.0
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.2
• 拓扑面积：
  133.71
• 氢给体数：
  1.0
• 氢受体数：
  7.0

安全信息

• 危险品标志：
  Xi
• 安全说明：
  S24/25
• WGK Germany：
  3
• 危险品运输编号：
  NONH for all modes of transport
• 海关编码：
  2934999090
• RTECS号：
  DJ1931000

制备方法与用途

生物活性

Nile Blue A (Nile blue sulfate) 可用于区分黑色素和脂褐素。它也可用于脂肪染色，以及制备电流葡萄糖传感器。

体外研究

Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule.

用途 

生物染色，区别脂肪酸和中性脂肪，病理组织中细菌和放线菌染色。也用于钽的光度测定。在中和分析中用以制备混合指示剂。

反应信息

• 作为反应物：
  描述：

  硫酸耐尔蓝 在 potassium bromate 、 硫酸 作用下， 生成 Nile blue sulfate
  参考文献：
  名称：

  Jonnalagadda, Sreekantha B.; Gollapalli, Nageswara R., South African Journal of Chemistry, 2001, vol. 54, p. 41 - 59

  摘要：

  DOI：

文献信息

• 620. The polarographic reduction of some natural and artificial hydrogen-carriers in bacterial enzyme systems
  作者：R. C. Kaye、H. I. Stonehill

  DOI：10.1039/jr9520003244

  日期：——