METHOD FOR MANUFACTURING MESENCHYMAL CELL LINE DERIVED FROM VERTEBRATE ANIMAL ADIPOSE TISSUE
申请人:Adiposeeds, Inc.
公开号:EP3385372A1
公开(公告)日:2018-10-10
It is to provide a method for producing a mesenchymal cell line derived from a vertebrate adipose tissue, and a mesenchymal cell line derived from a vertebrate adipose tissue produced by the production method. More specifically, an object of the present invention is to provide a method for producing a mesenchymal cell line derived from a vertebrate adipose tissue more simply, in a shorter period of time, and more efficiently, and a mesenchymal cell line derived from a vertebrate adipose tissue produced by the production method. The method for producing a mesenchymal cell line derived from a vertebrate adipose tissue of the present invention comprises the following step (A) and step (B): (A) inducing differentiation of one or more cells selected from a stromal vascular fraction comprising a mesenchymal stem cell, an adipose progenitor cell, and a stromal cell of a vertebrate adipose tissue into a mature adipocyte; and (B) inducing dedifferentiation of the mature adipocyte obtained in step (A) to obtain a mesenchymal cell line derived from the vertebrate adipose tissue;
本发明的目的是提供一种生产源自脊椎动物脂肪组织的间充质细胞系的方法,以及用该生产方法生产的源自脊椎动物脂肪组织的间充质细胞系。更具体地说,本发明的目的是提供一种更简单、更短时间、更高效率地生产源自脊椎动物脂肪组织的间充质细胞系的方法,以及用该生产方法生产的源自脊椎动物脂肪组织的间充质细胞系。本发明生产源自脊椎动物脂肪组织的间充质细胞系的方法包括以下步骤(A)和步骤(B):(A)将选自基质血管部分的一种或多种细胞诱导分化为成熟脂肪细胞,该基质血管部分包括间充质干细胞、脂肪祖细胞和脊椎动物脂肪组织的基质细胞;以及(B)诱导步骤(A)中获得的成熟脂肪细胞去分化,以获得源自脊椎动物脂肪组织的间充质细胞系;