申请人:Wadley Technologies, Inc.
公开号:EP0160743A2
公开(公告)日:1985-11-13
Methods and compositions are disclosed for the rapid identification of fungal pathogens. In a preferred method, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive medium, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter plating a sample of said visible fungi on miniature culture plates for example having the dimensions of about 33 x 75 x 5 millimeters, said plates containing differential fungal media, and thereafter indentifying fungal pathogens in a manner consistent with said medium selected.
In a related method utilizing the differential carbohydrate assimilation properties of fungal pathogens, a sample of human body fluid is obtained and subjected to analysis comprising inoculating a nutritive media, incubating said inoculum for such time and temperature as is necessary for visible fungal colony formation, thereafter inoculating a carbohydrate depleted medium with a sample of said visible fungi, incubating said inoculum for a period of time, thereafter plating a sample of said inoculated carbohydrate depleted medium on miniature culture plates, said plates containing differential carbohydrate assimilation media, and thereafter identifying said fungal pathogens in a manner consistent with said medium selected.
Novel compositions useful in practicing the disclosed methods are provided. These include an improved fungal growth medium, the improvement comprising the novel addition of ammonium ions to a medium comprising purified saponin, oxgall, a substrate for phenol oxidase and a supporting agent.
本发明公开了用于快速鉴定真菌病原体的方法和组合物。在一种优选的方法中,获取人体体液样本并进行分析,包括接种营养培养基,将所述接种物培养到可见真菌菌落形成所需的时间和温度,然后将所述可见真菌样本培养到微型培养板上,例如尺寸约为 33 x 75 x 5 毫米的培养板,所述培养板含有不同的真菌培养基,然后以与所选培养基一致的方式鉴定真菌病原体。
在一种利用真菌病原体的不同碳水化合物同化特性的相关方法中,获得人体体液样本并对其进行分析,包括接种营养培养基,将所述接种物培养到可见真菌菌落形成所需的时间和温度、然后将所述可见真菌样本接种到碳水化合物贫化培养基中,培养所述接种物一段时间,然后将所述接种的碳水化合物贫化培养基样本培养到微型培养板上,所述培养板含有不同的碳水化合物同化培养基,然后以与所选培养基一致的方式鉴定所述真菌病原体。
提供了有助于实施所公开方法的新型组合物。其中包括一种改良的真菌生长培养基,该改良包括向一种含有纯化皂素、羰基酚、酚氧化酶底物和支撑剂的培养基中添加铵离子。