sulfuric acid mono-(2-hydroxy-5-nitro-phenyl ester); monopotassium-salt | 10485-37-7

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机硫酸及其衍生物
• 英文名称: sulfuric acid mono-(2-hydroxy-5-nitro-phenyl ester); monopotassium-salt
• 英文别名: Schwefelsaeure-mono-(2-hydroxy-5-nitro-phenylester); Monokalium-Salz；Potassium-2-hydroxy-5-nitro-phenyl sulphate；potassium;(2-hydroxy-5-nitrophenyl) sulfate
• CAS: 10485-37-7
• 化学式: C₆H₄NO₇S*K
• 分子量: 273.265
• InChiKey: QKOKCLBGTOMYOV-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  -2.86
• 重原子数：
  16
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  141
• 氢给体数：
  1
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  sulfuric acid mono-(2-hydroxy-5-nitro-phenyl ester); monopotassium-salt 在 水 作用下， 生成 4-硝基儿茶酚
  参考文献：
  名称：

  Potentiometric Determination of Arylsulfatase Activity Using a Novel Nitrocatechol Sulfate PVC Membrane Sensor

  摘要：

  A novel potentiometric assay method for arylsulfatase enzyme is described based on measuring the initial reaction rate of hydrolysis of 4-nitrocatechol sulfate (4-NCS) substrate under optimized conditions. A monitoring sensor incorporating a PVC membrane with a nickel(II) bathophenanthroline-4-NCS ion-pair complex as electroactive material and 2-nitrophenyl phenyl ether as solvent mediator is developed and characterized. The sensor exhibits fast and stable linear response for 8 x 10(-3)-7.5 x 10(-6) M 4-NCS with an anionic slope of 58.5 +/- 0.2 mV/decade over the pH range 3-6, The sensor is used to follow up the decrease in a fixed concentration of 4-NCS (2 x 10(-4) M) as a function of arylsulfatase activity at pH 5.6 and 37 degrees C, A linear relationship between the initial rate of substrate hydrolysis and enzyme activity holds for 0.2-2.4 IU/mL (SD 2%). Activity measurement of arylsulfatase enzyme isolated from camel liver gives results that compare favorably wed with data obtained using the standard spectrophotometric assay method. Significant advantages over many of the previously described spectroscopic methods are offered by the proposed potentiometric technique.

  DOI：

  10.1021/ac00107a020

文献信息

• Potentiometric Determination of Arylsulfatase Activity Using a Novel Nitrocatechol Sulfate PVC Membrane Sensor
  作者：Saad S. M. Hassan、S. A. M. Marzouk、M. M. Abdel-Fattah、G. M. S. M. Shouckry

  DOI：10.1021/ac00107a020

  日期：1995.6.1

  A novel potentiometric assay method for arylsulfatase enzyme is described based on measuring the initial reaction rate of hydrolysis of 4-nitrocatechol sulfate (4-NCS) substrate under optimized conditions. A monitoring sensor incorporating a PVC membrane with a nickel(II) bathophenanthroline-4-NCS ion-pair complex as electroactive material and 2-nitrophenyl phenyl ether as solvent mediator is developed and characterized. The sensor exhibits fast and stable linear response for 8 x 10(-3)-7.5 x 10(-6) M 4-NCS with an anionic slope of 58.5 +/- 0.2 mV/decade over the pH range 3-6, The sensor is used to follow up the decrease in a fixed concentration of 4-NCS (2 x 10(-4) M) as a function of arylsulfatase activity at pH 5.6 and 37 degrees C, A linear relationship between the initial rate of substrate hydrolysis and enzyme activity holds for 0.2-2.4 IU/mL (SD 2%). Activity measurement of arylsulfatase enzyme isolated from camel liver gives results that compare favorably wed with data obtained using the standard spectrophotometric assay method. Significant advantages over many of the previously described spectroscopic methods are offered by the proposed potentiometric technique.