5-异硫氰酸基-3-氧代-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3',6'-二基二乙酸酯 | 118378-76-0

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 5-异硫氰酸基-3-氧代-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3',6'-二基二乙酸酯
• 英文名称: Fluorescein-diacetate-5-isothiocyanat
• 英文别名: (6'-acetyloxy-5-isothiocyanato-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate
• CAS: 118378-76-0
• 化学式: C₂₅H₁₅NO₇S
• 分子量: 473.463
• InChiKey: KUQQHHQNMMQOSV-UHFFFAOYSA-N

物化性质

• 沸点：
  685.1±55.0 °C(Predicted)
• 密度：
  1.44±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  5
• 重原子数：
  34
• 可旋转键数：
  5
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.12
• 拓扑面积：
  133
• 氢给体数：
  0
• 氢受体数：
  9

反应信息

• 作为反应物：
  描述：

  (1R,8S,9R,4E)-bicyclo[6.1.0]non-4-ene-9-ylmethyl (N-aminoethyl)carbamate 、 5-异硫氰酸基-3-氧代-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3',6'-二基二乙酸酯 以 二氯甲烷 为溶剂， 反应 1.5h， 以14.2 mg的产率得到(1R,8S,9R,4E)-bicyclo[6.1.0]non-4-ene-9-y-lmethyl (N-ethylthioureidofluorescein diacetate)carbamate
  参考文献：
  名称：

  Genetically Encoded Tetrazine Amino Acid Directs Rapid Site-Specific in Vivo Bioorthogonal Ligation with trans-Cyclooctenes

  摘要：

  Bioorthogonal ligation methods with improved reaction rates and less obtrusive components are needed for site-specifically labeling proteins without catalysts. Currently no general method exists for in vivo site-specific labeling of proteins that combines fast reaction rate with stable, nontoxic, and chemoselective reagents. To overcome these limitations, we have developed a tetrazine-containing amino acid, 1, that is stable inside living cells. We have site-specifically genetically encoded this unique amino acid in response to an amber codon allowing a single 1 to be placed at any location in a protein. We have demonstrated that protein containing 1 can be ligated to a conformationally strained trans-cyclooctene in vitro and in vivo with reaction rates significantly faster than most commonly used labeling methods.

  DOI：

  10.1021/ja2109745
• 作为产物：
  描述：

  Fluorescein isothiocyanate 、 乙酸酐 在 三乙胺 作用下， 以 二氯甲烷 为溶剂， 反应 1.5h， 生成 5-异硫氰酸基-3-氧代-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3',6'-二基二乙酸酯
  参考文献：
  名称：

  Genetically Encoded Tetrazine Amino Acid Directs Rapid Site-Specific in Vivo Bioorthogonal Ligation with trans-Cyclooctenes

  摘要：

  Bioorthogonal ligation methods with improved reaction rates and less obtrusive components are needed for site-specifically labeling proteins without catalysts. Currently no general method exists for in vivo site-specific labeling of proteins that combines fast reaction rate with stable, nontoxic, and chemoselective reagents. To overcome these limitations, we have developed a tetrazine-containing amino acid, 1, that is stable inside living cells. We have site-specifically genetically encoded this unique amino acid in response to an amber codon allowing a single 1 to be placed at any location in a protein. We have demonstrated that protein containing 1 can be ligated to a conformationally strained trans-cyclooctene in vitro and in vivo with reaction rates significantly faster than most commonly used labeling methods.

  DOI：

  10.1021/ja2109745

文献信息

• RADIATION DOSIMETRY SOL OR GEL AND RADIATION DOSIMETER COMPRISING SAME AS MATERIAL FOR RADIATION DOSIMETRY
  申请人：Riken

  公开号：EP3686632A1

  公开（公告）日：2020-07-29

  There is provided a radiation dosimetry sol or gel, and a radiation dosimeter containing the sol or the gel as a material for radiation dosimetry. A radiation dosimetry sol or gel characterized by comprising a compound (A) having an excitation light-induced fluorescence property that is changed by a radiolysis product of water; and a silicate salt (B). A radiation dosimetry sol or gel characterized by comprising a compound (A) having an excitation light-induced fluorescence property that is changed by a radiolysis product of water; a silicate salt (B); a water-soluble organic polymer (C) having an organic acid salt structure or an organic acid anion structure; and a dispersant (D) for the silicate salt (B). A radiation dosimeter comprising, as a material for radiation dosimetry, the above-mentioned radiation dosimetry sol or gel.

  提供了一种辐射剂量测定溶胶或凝胶，以及一种含有该溶胶或凝胶作为辐射剂量测定材料的辐射剂量计。 一种辐射剂量测定溶胶或凝胶，其特征在于包含一种化合物 (A)，该化合物具有由水的辐射分解产物改变的激发光诱导荧光特性；以及一种硅酸盐 (B)。 一种辐射剂量计溶胶或凝胶，其特征在于包含一种化合物（A），该化合物具有受水的辐射分解产物影响而改变的激发光诱导荧光特性；一种硅酸盐（B）；一种具有有机酸盐结构或有机酸阴离子结构的水溶性有机聚合物（C）；以及一种用于硅酸盐（B）的分散剂（D）。 一种辐射剂量计，包括作为辐射剂量测定材料的上述辐射剂量测定溶胶或凝胶。
• Radiation dosimetry sol or gel and radiation dosimeter comprising same as material for radiation dosimetry
  申请人：RIKEN

  公开号：US11150358B2

  公开（公告）日：2021-10-19

  A radiation dosimetry sol or gel, and a radiation dosimeter containing the sol or the gel as a material for radiation dosimetry. A radiation dosimetry sol or gel including a compound (A) having an excitation light-induced fluorescence property that is changed by a radiolysis product of water; and a silicate salt (B). A radiation dosimetry sol or gel including a compound (A) having an excitation light-induced fluorescence property that is changed by a radiolysis product of water; a silicate salt (B); a water-soluble organic polymer (C) having an organic acid salt structure or an organic acid anion structure; and a dispersant (D) for the silicate salt (B). A radiation dosimeter including, as a material for radiation dosimetry, the above-mentioned radiation dosimetry sol or gel.

  一种辐射剂量测定溶胶或凝胶，以及一种含有该溶胶或凝胶作为辐射剂量测定材料的辐射剂量计。一种辐射剂量测定溶胶或凝胶，包括一种化合物 (A)，其激发光诱导荧光特性会因水的辐射分解产物而改变；以及一种硅酸盐 (B)。一种辐射剂量测定溶胶或凝胶，包括一种化合物（A），其激发光诱导荧光特性会因水的辐射分解产物而改变；一种硅酸盐（B）；一种水溶性有机聚合物（C），其具有有机酸盐结构或有机酸阴离子结构；以及一种用于硅酸盐（B）的分散剂（D）。一种辐射剂量计，包括作为辐射剂量测定材料的上述辐射剂量测定溶胶或凝胶。
• SIGNAL AMPLIFICATION MICROSPHERES, THEIR USE IN ONE-STEP AND MULTI-STEP ANALYTICAL AMPLIFICATION PROCEDURES AND METHODS FOR THEIR PRODUCTION
  申请人：Supernova Diagnostics, Inc.

  公开号：EP2440926A1

  公开（公告）日：2012-04-18
• Methods of production of signal amplification microspheres
  申请人：Supernova Diagnostics, Inc.

  公开号：EP2440926B1

  公开（公告）日：2014-12-10
• SELF INDICATING RADIATION ALERT DOSIMETER
  申请人：Patel Gordhabhai N.

  公开号：US20120205591A1

  公开（公告）日：2012-08-16

  Described is a self-indicating instant radiation dosimeter ( 1001 ) for monitoring high energy radiations, such as X-ray. The dosimeter contains a radiation sensitive, color changing, indicating composition ( 10 ), e.g., a diacetylene (R—C≡C—C≡C—R′, where R and R′ are substituents groups) or a radiochromic dye, a polymeric binder ( 20 ) and optionally a shelf life extender ( 50 ) or an activator ( 40 ). The radiation sensitive composition ( 10 ) changes color instantly when exposed to high energy radiation. The dose is estimated by comparing the color with a color reference chart or more accurately with a spectrophotometer or an optical densitometer. The radiation sensitive composition ( 10 ) is protected from low energy radiation such as UV light, by a layer of low energy absorbing materials, such as UV absorbers.