4-amino-1-[(2R,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-2-oxopyrimidine-5-carboxylic acid

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 4-amino-1-[(2R,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-2-oxopyrimidine-5-carboxylic acid
• 化学式: C₁₀H₁₃N₃O₆
• 分子量: 271.23
• InChiKey: FHPQEVWDHUHVGT-JXBXZBNISA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.5
• 重原子数：
  19
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  146
• 氢给体数：
  4
• 氢受体数：
  6

文献信息

• REAGENT FOR MASS SPECTROMETRY
  申请人：Roche Diagnostics Operations, Inc.

  公开号：US20200041470A1

  公开（公告）日：2020-02-06

  The present invention relates to reagents suitable in the mass spectrometric determination of analyte molecules such as carbohydrates as well as adducts of such reagents and analyte molecules and applications of said reagents and adducts. Further, the present invention relates to methods for the mass spectrometric determination of analyte molecules.

  本发明涉及适用于质谱测定分析物分子（如碳水化合物）以及该类试剂和分析物分子的加合物的试剂，并涉及所述试剂和加合物的应用。此外，本发明涉及用于质谱测定分析物分子的方法。
• Methods for detection of nucleotide modification
  申请人：Cambridge Epigenetix Limited

  公开号：US10428381B2

  公开（公告）日：2019-10-01

  This invention relates to the identification of modified cytosine residues, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) to be distinguished from cytosine (C) in a sample nucleotide sequence. Methods may comprise oxidizing or reducing a first portion of polynucleotides which comprise the sample nucleotide sequence; treating the oxidized or reduced first portion and a second portion of polynucleotides with bisulfite; sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and; identifying the residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence. These methods may be useful, for example in the analysis of genomic DNA and/or of RNA.

  本发明涉及鉴定修饰胞嘧啶残基，如 5-甲基胞嘧啶（5mC）、5-羟甲基胞嘧啶（5hmC）和 5-甲酰基胞嘧啶（5fC），以便与样本核苷酸序列中的胞嘧啶（C）区分开来。方法可包括氧化或还原包含样本核苷酸序列的第一部分多核苷酸；用亚硫酸氢盐处理氧化或还原的第一部分和第二部分多核苷酸；按照步骤 ii) 和 iii) 对第一部分和第二部分群体中的多核苷酸进行测序，分别产生第一和第二核苷酸序列；鉴定第一和第二核苷酸序列中与样本核苷酸序列中胞嘧啶残基相对应的残基。这些方法可用于基因组 DNA 和/或 RNA 的分析等。
• Method for modifying a template double stranded polynucleotide using a MuA transposase
  申请人：Oxford Nanopore Technologies Ltd.

  公开号：US10570440B2

  公开（公告）日：2020-02-25

  The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

  本发明涉及一种修饰模板双链多核苷酸的方法，特别是用于利用纳米孔测序进行表征。该方法可从模板中产生多个经修饰的双链多核苷酸。然后可以对这些修饰的多核苷酸进行表征。
• Sample preparation method
  申请人：Oxford Nanopore Technologies Ltd.

  公开号：US10669578B2

  公开（公告）日：2020-06-02

  The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.

  本发明涉及一种表征模板多核苷酸的改进方法。该方法包括使用聚合酶制备修饰的多核苷酸，使其比模板多核苷酸更容易表征。
• Methods and systems for identifying candidate nucleic acid agent
  申请人：APTITUDE MEDICAL SYSTEMS, INC.

  公开号：US11041849B2

  公开（公告）日：2021-06-22

  The present disclosure provides methods, kits and compositions for identifying nucleic acid agents having a desired property, e.g., a property of specifically binding to a target (such as a protein target) with high affinity. More specifically, the present disclosure provides methods, kits and compositions for identifying candidate nucleic acid agents with both high specificity and affinity for a target.

  本公开提供了用于鉴定具有所需特性的核酸制剂的方法、试剂盒和组合物，例如，与靶标（如蛋白质靶标）高亲和力特异性结合的特性。更具体地说，本公开内容提供了用于鉴定对靶标具有高特异性和高亲和性的候选核酸制剂的方法、试剂盒和组合物。