[EN] A LIBRARY OF MODIFIED PRIMERS FOR NUCLEIC ACID SEQUENCING, AND METHOD OF USE THEREOF<br/>[FR] BIBLIOTHEQUE D'AMORCES MODIFIEES POUR LE SEQUENÇAGE D'ACIDE NUCLEIQUE ET METHODE D'UTILISATION DE LADITE BIBLIOTHEQUE
申请人:SEQUETECH CORP
公开号:WO2000028087A1
公开(公告)日:2000-05-18
Modified oligonucleotide primers, libraries of such primers and methods for their use in characterizing a selected target sequence, via a polymerase-catalyzed primer-extension reaction is described. The primers are from 7-11 bases in length, have a Tm greater than or equal to 35 °C and are effective for use in characterizing a selected target which has a known sequence of at least about 100 nucleotides. A method of selecting a primer from a ready-made reduced-set library of such oligonucleotides using a computer interface is also described.