2,2,2-trifluoro-N-[3-[4-[(1S)-1-[[1-[(2R,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-2,4-dioxopyrimidin-5-yl]methoxy]-2-methylpropyl]-3-nitrophenyl]prop-2-ynyl]acetamide

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 2,2,2-trifluoro-N-[3-[4-[(1S)-1-[[1-[(2R,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-2,4-dioxopyrimidin-5-yl]methoxy]-2-methylpropyl]-3-nitrophenyl]prop-2-ynyl]acetamide
• 化学式: C25H27F3N4O9
• 分子量: 584.5
• InChiKey: QGSFROANTGIFTF-VKEMMKHGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.6
• 重原子数：
  41
• 可旋转键数：
  9
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.48
• 拓扑面积：
  183
• 氢给体数：
  4
• 氢受体数：
  12

文献信息

• Photocleavable labeled nucleotides and nucleosides and methods for their use in DNA sequencing
  申请人：Wu Weidong

  公开号：US20080132692A1

  公开（公告）日：2008-06-05

  Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.

  本文提供了新型核苷酸、核苷和它们的衍生物，可以用于DNA测序技术和其他类型的DNA分析。在一种实施方式中，核苷酸或核苷的3'-OH基团未保护，通过连接剂连接到光致解终止基团的荧光染料，衍生化到核碱基上。光致解荧光基团被设计成能够终止DNA合成，并被裂解，以便在并行格式下高效地测序DNA寡聚物。在核苷酸和核苷上设计这种快速可裂解的荧光基团，可以增强大型DNA寡聚物并行测序的速度和准确性，以实现快速全基因组测序和多态性等有价值的遗传信息的识别，同时在裂解荧光基团后，还可以对核酸分子进行进一步的操作和分析。
• US7897737B2
  申请人：——

  公开号：US7897737B2

  公开（公告）日：2011-03-01