5-(bromoacetamido)fluorescein | 107142-62-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 5-(bromoacetamido)fluorescein
• 英文别名: 2-bromo-N-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)acetamide
• CAS: 107142-62-1
• 化学式: C₂₂H₁₄BrNO₆
• 分子量: 468.26
• InChiKey: VWSZZEWXEIXKLJ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.4
• 重原子数：
  30
• 可旋转键数：
  2
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.09
• 拓扑面积：
  105
• 氢给体数：
  3
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  5-(bromoacetamido)fluorescein 、 7,8-dihydromorphine-3-(2 aminoethyl) ether hydrochloride 在 三乙胺 作用下， 以 N-甲基乙酰胺 、 甲醇 为溶剂， 生成 7,8-Dihydromorphine-3-[2-(fluorescein-5-ylamino carbonylmethyleneamino)ethyl] Ether
  参考文献：
  名称：

  Immunoassay for opiate alkaloids and their metabolites; tracers,

  摘要：

  本发明涉及一种用于鸦片生物碱及其代谢产物的荧光偏振测定法，以及制备和执行该测定法所需的各种组分和制备这些组分的方法。具体地，本发明揭示了追踪剂、免疫原和抗体，以及制备它们的方法。追踪剂和免疫原是由取代鸦片生物碱制成的。追踪剂中包括荧光素基团，而免疫原中则包括一种聚氨基酸。该测定法是通过测量样品与抗血清和追踪剂混合后，用平面偏振光照射时产生的荧光的偏振保留程度来进行的。

  公开号：

  US05258512A1

文献信息

• Procainamide assay, tracers, immunogens and antibodies
  申请人：ABBOTT LABORATORIES

  公开号：EP0199042A1

  公开（公告）日：1986-10-29

  The present invention is directed to a fluorescence polarization immunoassay for procainamide, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from analogs of procainamide. A fluorescein moiety is included in the tracer while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of ' polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

  该发明涉及一种用于普鲁卡因胺的荧光偏振免疫分析，以及制备和执行此类分析所需的各种组分，以及制造这些组分的方法。具体地，揭示了示踪剂、免疫原和抗体，以及制造它们的方法。示踪剂和免疫原是由普鲁卡因胺的类似物制成的。示踪剂中包含荧光基团，而多肽（氨基酸）是免疫原的一部分。该分析通过测量通过含有抗血清和示踪剂的样品的偏振保留度来进行，该样品已经通过平面偏振光。
• Tracers for use in flecainide fluorescence polarization immunoassay
  申请人：ABBOTT LABORATORIES

  公开号：EP0242847A2

  公开（公告）日：1987-10-28

  The present invention is directed to a fluorescence polarization assay for determining the amount of flecainide in fluids, especially biological fluids such as serum, plasma or urine. The present invention also relates to novel tracer compounds useful in fluorescence polarization assay techniques.

  本发明涉及一种荧光偏振测定法，用于确定体液，特别是血清、血浆或尿液等生物体液中的非加尼含量。 本发明还涉及可用于荧光偏振测定技术的新型示踪化合物。
• Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies
  申请人：ABBOTT LABORATORIES

  公开号：EP0254120A2

  公开（公告）日：1988-01-27

  The present invention is directed to a fluorescence polarization assay for opiate alkaloids and their metabolites, to the various components needed for preparing and carrying out such an assay and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted opiate alkaloids. A fluorescein moiety is included in the tracers, while a poly(amino acid) forms a part of the immunogens. The assay is conducted by measuring the degree of polarization retention of the fluorescence resulting when a sample mixed with antiserum and tracer is irradiated with plane-polarized light.

  本发明涉及鸦片生物碱及其代谢物的荧光偏振测定法、制备和进行这种测定法所需的各种成分以及制造这些成分的方法。 具体地说，本发明公开了示踪剂、免疫原和抗体以及制造它们的方法。 示踪剂和免疫原是由取代的鸦片生物碱制成的。 示踪剂中含有荧光素分子，而免疫原中含有聚氨基酸。 该测定法是通过测量当用平面偏振光照射与抗血清和示踪剂混合的样品时所产生的荧光的偏振保留程度来进行的。
• US4939264A
  申请人：——

  公开号：US4939264A

  公开（公告）日：1990-07-03
• US5258512A
  申请人：——

  公开号：US5258512A

  公开（公告）日：1993-11-02