申请人:Ikeda Masato
公开号:US20050130276A1
公开(公告)日:2005-06-16
The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.
本发明提供了一个
多肽,其包括从属于Coryneform细菌的微
生物中获得的异丙基
丙酸异构酶的
氨基酸序列中至少删除,替换或添加了至少一个
氨基酸,其中生产包含该
氨基酸序列作为唯一异丙基
丙酸异构酶的Coryneform细菌在最小
培养基中表现出部分亮
氨酸需求;编码该
多肽的DNA,包含该DNA的
重组DNA,转化了
重组DNA的微
生物以及使用该微
生物生产L-
氨基酸的方法。