申请人:Kyowa Hakko Kogyo Co., Ltd.
公开号:US07445915B2
公开(公告)日:2008-11-04
The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.
本发明提供了一种多肽,包括从属于Coryneform细菌的微生物中获得的异丙基丙酸异构酶的氨基酸序列中至少删除,替换或添加一个氨基酸的氨基酸序列。其中,生产包含该氨基酸序列作为唯一异丙基丙酸异构酶的多肽的Coryneform细菌在最小培养基中表现出部分亮氨酸需求。本发明还提供了编码该多肽的DNA,包含该DNA的重组DNA,转化有重组DNA的微生物以及使用该微生物生产L-氨基酸的方法。