Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
申请人:Weiner David P.
公开号:US08709772B2
公开(公告)日:2014-04-29
This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., damino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H2O+acceptor<=>a 2-oxo acid+NH3+reduced acceptor. Thus, the invention provides enzymes, compositions, methods for production of pharmaceutical compositions, pharmaceutical intermediates, antibiotics, sweeteners, peptide enzymes, peptide hormones, fuel and fuel additive compositions, foods and food additives, beverage and beverage additives, feeds and feed additives, drugs and drug additives, dietary supplements, textiles, wood, paper, pulp, and detergents comprising the polypeptides or polynucleotides in accordance with the invention.
本发明涉及酶、编码酶的多核苷酸、使用这种多核苷酸和多肽以及更具体地涉及具有转移酶活性的酶,例如转氨酶活性,例如D-氨基酸转移酶活性,和/或氧化还原酶活性,例如脱氢酶活性,例如D-氨基酸脱氢酶活性,并/或催化化学基团的转移,催化转氨作用,催化反应:D-丙氨酸+2-酮戊二酸<=>丙酮酸+D-谷氨酸,并/或催化氧化还原反应,催化氢原子的去除,并/或催化反应:D-氨基酸+H2O+受体<=>2-酮酸+NH3+还原的受体。因此,本发明提供了酶、组成物、制药组分的生产方法、制药中间体、抗生素、甜味剂、肽酶、肽激素、燃料和燃料添加剂组合物、食品和食品添加剂、饮料和饮料添加剂、饲料和饲料添加剂、药物和药物添加剂、膳食补充剂、纺织品、木材、纸张、纸浆和洗涤剂,其中包括符合本发明的多肽或多核苷酸。