(E)-3-{1'-benzyl-3,4-dihydro-4-oxospiro[2H-(1,3)-benzoxazine-2,4'-piperidin]-6-yl}-N-hydroxyacrylamide hydrochloride

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 哌啶类
• 英文名称: (E)-3-{1'-benzyl-3,4-dihydro-4-oxospiro[2H-(1,3)-benzoxazine-2,4'-piperidin]-6-yl}-N-hydroxyacrylamide hydrochloride
• 英文别名: (E)-3-(1'-benzyl-4-oxospiro[3H-1,3-benzoxazine-2,4'-piperidine]-6-yl)-N-hydroxyprop-2-enamide;hydrochloride
• 化学式: C₂₂H₂₃N₃O₄*ClH
• 分子量: 429.903
• InChiKey: BEMRUJYWMJEERN-BXTVWIJMSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.74
• 重原子数：
  30
• 可旋转键数：
  4
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.27
• 拓扑面积：
  90.9
• 氢给体数：
  4
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  5-溴水杨酰胺 在 四氢吡咯 、 盐酸 、 水 、 palladium diacetate 、 1-羟基苯并三唑 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 三乙胺 、 三(邻甲基苯基)磷 、 sodium hydroxide 作用下， 以 1,4-二氧六环 、 甲醇 、 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 25.0h， 生成 (E)-3-{1'-benzyl-3,4-dihydro-4-oxospiro[2H-(1,3)-benzoxazine-2,4'-piperidin]-6-yl}-N-hydroxyacrylamide hydrochloride
  参考文献：
  名称：

  Synthesis and biological characterization of spiro[2H-(1,3)-benzoxazine-2,4′-piperidine] based histone deacetylase inhibitors

  摘要：

  Histone Deacetylases (HDACs) have become important targets for the treatment of cancer and other diseases. In previous studies we described the development of novel spirocyclic HDAC inhibitors based on the combination of privileged structures with hydroxamic acid moieties as zinc binding group. Herein, we report further explorations, which resulted in the discovery of a new class of spiro[2H-(1,3)-benzoxazine-2,4'-piperidine] derivatives. Several compounds showed good potency of around 100 nM and less in the HDAC inhibition assays, submicromolar IC50 values when tested against tumour cell lines and a remarkable stability in human and mouse microsomes. Two representative examples exhibited a good pharmacokinetic profile with an oral bioavailability equal or higher than 35% and one of them studied in an HCT116 murine xenograft model showing a robust tumour growth inhibition. In addition, the two benzoxazines were found to have a minor affinity for the hERG potassium channel compared to their corresponding ketone analogues. (C) 2013 Elsevier Masson SAS. All rights reserved.

  DOI：

  10.1016/j.ejmech.2013.03.061

文献信息

• Synthesis and biological characterization of spiro[2H-(1,3)-benzoxazine-2,4′-piperidine] based histone deacetylase inhibitors
  作者：Florian Thaler、Mario Varasi、Agnese Abate、Giacomo Carenzi、Andrea Colombo、Chiara Bigogno、Roberto Boggio、Roberto Dal Zuffo、Daniela Rapetti、Anna Resconi、Nickolas Regalia、Stefania Vultaggio、Giulio Dondio、Stefania Gagliardi、Saverio Minucci、Ciro Mercurio

  DOI：10.1016/j.ejmech.2013.03.061

  日期：2013.6

  Histone Deacetylases (HDACs) have become important targets for the treatment of cancer and other diseases. In previous studies we described the development of novel spirocyclic HDAC inhibitors based on the combination of privileged structures with hydroxamic acid moieties as zinc binding group. Herein, we report further explorations, which resulted in the discovery of a new class of spiro[2H-(1,3)-benzoxazine-2,4'-piperidine] derivatives. Several compounds showed good potency of around 100 nM and less in the HDAC inhibition assays, submicromolar IC50 values when tested against tumour cell lines and a remarkable stability in human and mouse microsomes. Two representative examples exhibited a good pharmacokinetic profile with an oral bioavailability equal or higher than 35% and one of them studied in an HCT116 murine xenograft model showing a robust tumour growth inhibition. In addition, the two benzoxazines were found to have a minor affinity for the hERG potassium channel compared to their corresponding ketone analogues. (C) 2013 Elsevier Masson SAS. All rights reserved.