4-nitro-1-naphthyl phosphate | 60840-06-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: 4-nitro-1-naphthyl phosphate
• 英文别名: 4NNPP；4-Nitro-1-naphthylphosphat-monoester；(4-Nitronaphthalen-1-yl) dihydrogen phosphate
• CAS: 60840-06-4
• 化学式: C₁₀H₈NO₆P
• 分子量: 269.15
• InChiKey: RQQFEJKTXYWTAZ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.3
• 重原子数：
  18
• 可旋转键数：
  2
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  113
• 氢给体数：
  2
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  4-nitro-1-naphthyl phosphate 在 calf intestine alkaline phosphatase 作用下， 以 aq. buffer 为溶剂， 生成 4-硝基-1-萘酚
  参考文献：
  名称：

  用于中性或弱酸性最佳pH值的水解酶的4-硝基-1-萘酚的生色底物：以4-硝基-1-萘基-β-d-吡喃半乳糖苷为例

  摘要：

  在pH为5.5至7.6，在450nm 4-硝基-1-萘酚的吸收率是超过其的2.1倍对在405nm和硝基苯酚超过该9.6倍邻在415nm处硝基苯酚。上-4-硝基-1-萘基-β- d -galactopyranoside在pH 7.4，催化效率大肠杆菌β- d半乳糖苷酶是3倍的，关于对硝基苯基β- d -galactopyranoside和约40的那％在邻硝基苯-β- d上-半乳糖吡喃糖苷，并通过酶联免疫吸附测定法产生较低的青霉素G定量限。因此，4-硝基-1-萘酚有利于制备中性或弱酸性最佳pH的水解酶的生色底物。

  DOI：

  10.1016/j.bmcl.2012.11.120

文献信息

• Integral multilayer analytical element for measurement of alkaline phosphatase activity
  申请人：FUJI PHOTO FILM CO., LTD.

  公开号：EP0205950A1

  公开（公告）日：1986-12-30

  An integral multilayer analytical element for the measurement of alkaline phosphatase activity, which comprises a porous spreading layer containing a self-developable substrate in response to contact with alkaline phosphatase, a buffer layer containing a diffusion-resistant base and a support in a laminated form.

  一种用于测量碱性磷酸酶活性的整体多层分析元件，它包括一个多孔铺展层，内含与碱性磷酸酶接触后可自动显影的基底；一个缓冲层，内含抗扩散基底；以及一个层叠形式的支撑物。
• [EN] METHOD FOR SIMULTANEOUSLY MEASURING ACTIVITY OF VARIOUS ENZYMES BY USING MULTI-WAVELENGTH ABSORPTION SINGLE CHANNEL<br/>[FR] PROCÉDÉ DE MESURE SIMULTANÉE DE L'ACTIVITÉ DE DIFFÉRENTS ENZYMES EN UTILISANT UN CANAL UNIQUE D'ABSORPTION DE LONGUEURS D'ONDE MULTIPLES
  申请人：UNIV CHONGQING MEDICAL

  公开号：WO2014043923A1

  公开（公告）日：2014-03-27

  本发明公开了一种用多波长吸收单通道同步测量多种酶活性的方法；基于光吸收线性加和性及酶显色底物与其对应显色产物的等吸收波长，建立多波长吸收同步测量单通道同步测定多种酶活性所需显色底物组合设计原理及消除吸收光谱重叠干扰的数据处理方法，并以数值积分的速度方程分析酶反应过程测定最大反应速度消除各种底物及产物对待测酶活性的干扰，使单通道同步测定多种酶活性的范围及检测限都与单独测定时相当；该方法可用于同步测定生物样品多种酶活性、酶标记免疫分析同步测定多组分含量、同步筛选不同靶酶的抑制剂，可用于临床生化分析和临床免疫检验、卫生检验、酶抑制剂筛选等常规实践，也可用于这类酶法分析技术适用的各种基础研究。
• METHOD FOR SIMULTANEOUSLY MEASURING ACTIVITY OF VARIOUS ENZYMES BY USING MULTI-WAVELENGTH ABSORPTION SINGLE CHANNEL
  申请人：CHONGQING MEDICAL UNIVERSITY

  公开号：US20150225764A1

  公开（公告）日：2015-08-13

  A method for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances is claimed; based on linear additivity of absorbances and the isoabsorbance wavelengths of chromogenic substrates and their chromogenic products, both the principles for the combination of chromogenic substrates required for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances and an approach for data processing to eliminate the interference of the overlapped absorbances are developed, kinetic analysis of reaction curves via numerical integration eliminates the interference of all substrates and products and estimates the maximal reaction rates of the enzymes to be measured, giving the linear ranges and the limits of quantification for simultaneously measuring the activities of multiple kinds of enzymes in single channel comparable to those by separate assays; the present invention is applicable for simultaneously measuring the activities of multiple kinds of enzymes in biological samples, simultaneously measuring multiple components by enzyme-labeled immunoassays, screening simultaneously of inhibitors against multiple targets, for the practice in clinical biochemical analyses, clinical immunoassays, health laboratory analyses, the discovery of inhibitors, and the basical researches which need the present invention.
• US4839278A
  申请人：——

  公开号：US4839278A

  公开（公告）日：1989-06-13
• US9611502B2
  申请人：——

  公开号：US9611502B2

  公开（公告）日：2017-04-04