5(6)-羧基-2',7'-二氯荧光素 | 111843-78-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 5(6)-羧基-2',7'-二氯荧光素
• 英文名称: CDCF
• 英文别名: 5(6)-carboxy-2′,7′-dichlorofluorescein；5-carboxy-2′,7′-dichloroflourescein；5-carboxy-2′,7′-dichlorofluorescein；5(6)-carboxy-2',7'-dichlorofluorescein；5-carboxy-2',7'-dichlorofluorescein；2',7'-dichloro-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• CAS: 111843-78-8
• 化学式: C₂₁H₁₀Cl₂O₇
• 分子量: 445.212
• InChiKey: JGZVUTYDEVUNMK-UHFFFAOYSA-N

物化性质

• 熔点：
  ≥250°C (lit.)
• 溶解度：
  在DMF中可溶

计算性质

• 辛醇/水分配系数(LogP)：
  4.67
• 重原子数：
  30.0
• 可旋转键数：
  1.0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  113.29
• 氢给体数：
  3.0
• 氢受体数：
  6.0

反应信息

• 作为产物：
  描述：

  trimellitic anhydride 、 4-氯间苯二酚 在 甲烷磺酸 作用下， 以 水 为溶剂， 生成 5(6)-羧基-2',7'-二氯荧光素
  参考文献：
  名称：

  INTRACELLULAR ORGANELLE PEPTIDE TARGETED ENZYME SUBSTRATES

  摘要：

  该发明涉及基于细胞器酶活性的底物和方法，用于可视化细胞内细胞器，如溶酶体、过氧化物酶体、细胞核、内质网和高尔基体。这些化合物代表了具有化学上不同的酶底物与靶向和检测底物的新型组合，这些底物受到靶细胞器内的酶活性的激活而产生可检测信号。该发明的细胞器靶向酶底物旨在在一些细胞器中发现的较低pH值下提供高荧光，并可用于监测细胞内酶活性的非常低浓度。

  公开号：

  US20150219654A1

文献信息

• Method for counting living cells of microbes and apparatus therefor
  申请人：MITSUBISHI JUKOGYO KABUSHIKI KAISHA

  公开号：EP0443700A2

  公开（公告）日：1991-08-28

  A method for counting living cells of microbes in a fluid sample continuously while flowing the sample using an apparatus which comprises a system for supplying at a predetermined rate to the flow line of the sample a reagent, such as, a derivative of fluorescein, capable of reacting with one or more substances intrinsic of the living cell, such as enzymes, to form an accumulative fluorescent product within the living cells; a reactor inserted in said flow line of the sample and being provided for said reaction of the reagent with the cell-intrinsic substance in the living cells; a photometric detection system arranged subsequent to said reactor for detecting fluorescence emitted as individual luminous point from the fluorescent product in each of the living cells floating in the flowing sample upon irradiation of the fluorescent product by an exciting ray; and an electronic unit including a pulse counter for counting electric pulses produced from each fluorescence from the luminous point.

  一种在流体样品流动过程中连续计数流体样品中微生物活细胞的方法，该方法使用一种设备，该设备包括一个系统，该系统以预定速率向样品的流线提供一种试剂，如荧光素的衍生物，该试剂能够与活细胞中的一种或多种固有物质（如酶）发生反应，在活细胞内形成累积荧光产物；一个反应器，插入所述样品流管中，用于所述试剂与活细胞中的细胞固有物质发生反应；一个光度检测系统，布置在所述反应器之后，用于检测浮在流动样品中的每个活细胞中的荧光产物在受激光线照射后作为单个发光点发出的荧光；以及一个电子装置，包括一个脉冲计数器，用于计数每个发光点发出的荧光产生的电脉冲。
• METHOD FOR CULTURE OF ANIMAL HEPATOCYTE
  申请人：Hitachi, Ltd.

  公开号：EP2383332A1

  公开（公告）日：2011-11-02

  Provided are a technique for easily forming a spheroid by three-dimensionally culturing hepatocytes, and a technique for forming a spheroid having a higher expression level of a transporter MRP2 playing a role of biliary excretion than that of a conventional method. In order to solve the above-described problems, the present inventors have found out a condition under which hepatocytes easily form the spheroid on a nanopillar sheet. More specifically, this is related to a concentration of Type I collagen coated onto the NP sheet. Also, they have found out a condition under which an expression level of a gene related to the excretion of the formed spheroid is improved. More specifically, after the spheroid is previously formed, a biological matrix is overlayered thereon.

  本发明提供了一种通过三维培养肝细胞轻松形成球形体的技术，以及一种与传统方法相比具有更高表达水平的起胆汁排泄作用的转运体 MRP2 的球形体形成技术。为了解决上述问题，本发明人发现了一种条件，在这种条件下，肝细胞很容易在纳米柱薄片上形成球体。更具体地说，这与涂在纳米柱薄片上的 I 型胶原蛋白的浓度有关。此外，他们还发现了一种条件，在这种条件下，与已形成的小球的排泄有关的基因的表达水平会得到改善。更具体地说，在先前形成球体后，在其上覆盖一层生物基质。
• Methods for detecting film-forming amines in water
  申请人：CHEMTREAT INC.

  公开号：US11016027B2

  公开（公告）日：2021-05-25

  Methods and systems for detecting and quantifying film forming amines in water systems are described. Water containing a film forming amine is combined with a fluorescent dye, the pH of the water is reduced, and then the fluorescence signal of the fluorescent dye is measured. The fluorescence signal is indicative of the amount of film forming amine in the water, and can be used to quantify the amount of film forming amine and control the amount of film forming amine that is introduced into the water system.

  本文介绍了检测和量化水系统中成膜胺的方法和系统。将含有成膜胺的水与荧光染料混合，降低水的 pH 值，然后测量荧光染料的荧光信号。荧光信号可指示水中成膜胺的含量，可用于量化成膜胺的含量并控制引入水系统的成膜胺的含量。
• Use of fluorescent reagents in identification of cancerous cells and activated lymphocytes
  申请人：——

  公开号：US20010049091A1

  公开（公告）日：2001-12-06

  The present invention provides methods of determining whether test cells in a sample are cancerous or not and determining if lymphocytes are activated or not. The method measures the test cell's DNA ploidy and the cellular activity of an enzyme such as an esterase, which has altered expression in cancer cells. Esterase activity can be measured using fluorescent compounds such as fluorescein diacetate.

  本发明提供了确定样本中的测试细胞是否癌变以及确定淋巴细胞是否活化的方法。该方法可测量检测细胞的 DNA 倍性和一种酶（如酯酶）的细胞活性，酯酶在癌细胞中的表达会发生改变。酯酶活性可通过荧光化合物（如二乙酸荧光素）来测量。
• Fluorescent dyed lubricant for medical devices
  申请人：——

  公开号：US20030018353A1

  公开（公告）日：2003-01-23

  A composition and method for detecting the presence, location and uniformity of a lubricious coating on a medical device. The method includes the steps of preparing a mixture of a biocompatible hydrophilic fluorescent dye and a hydrophobic lubricant, applying the mixture to the surface of a medical device to form a coating capable of exhibiting fluorescence, and subjecting the surface of the medical device to a source of energy capable of inducing a fluorescent emission. The coating composition may further comprise a surfactant. This method finds particular utility for catheters, especially stent delivery catheters.

  一种用于检测医疗器械上润滑涂层的存在、位置和均匀性的组合物和方法。该方法包括以下步骤：制备生物相容性亲水性荧光染料和疏水性润滑剂的混合物，将该混合物涂抹到医疗设备表面以形成能够发出荧光的涂层，以及将医疗设备表面置于能够诱发荧光发射的能量源下。涂层组合物可进一步包含表面活性剂。这种方法特别适用于导管，尤其是支架输送导管。