1,4-Bis(azidomethyl)piperazine | 1209493-89-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二嗪烷类
• 英文名称: 1,4-Bis(azidomethyl)piperazine
• CAS: 1209493-89-9
• 化学式: C₆H₁₂N₈
• 分子量: 196.215
• InChiKey: FAWKVQJYKRLPJO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.1
• 重原子数：
  14
• 可旋转键数：
  4
• 环数：
  1.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  35.2
• 氢给体数：
  0
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  1,4-Bis(azidomethyl)piperazine 、 丁炔二酸二甲酯 以 乙醚 为溶剂， 以87%的产率得到
  参考文献：
  名称：

  Cyanomethylamines and azidomethylamines: new general methods of the synthesis and transformations

  摘要：

  Simple and efficient methods have been developed to obtain cyanomethylamines and azidomethylamines using reactions of methoxymethylamines with TMSCN and TMSN3, respectively. In the case of dimethylformamide dimethylacetal, only one MeO group was substituted with CN, and an unexpected direction of the subsequent azidation was found. Adducts of azidomethylamines with DMAD were studied, and the base-catalyzed isomerization of symmetric triazoles into non-symmetric ones was revealed.

  DOI：

  10.1016/j.mencom.2009.09.018
• 作为产物：
  描述：

  叠氮基三甲基硅烷 、 双(甲氧基甲基)哌嗪 以 乙醚 为溶剂， 以91.5%的产率得到1,4-Bis(azidomethyl)piperazine
  参考文献：
  名称：

  Cyanomethylamines and azidomethylamines: new general methods of the synthesis and transformations

  摘要：

  Simple and efficient methods have been developed to obtain cyanomethylamines and azidomethylamines using reactions of methoxymethylamines with TMSCN and TMSN3, respectively. In the case of dimethylformamide dimethylacetal, only one MeO group was substituted with CN, and an unexpected direction of the subsequent azidation was found. Adducts of azidomethylamines with DMAD were studied, and the base-catalyzed isomerization of symmetric triazoles into non-symmetric ones was revealed.

  DOI：

  10.1016/j.mencom.2009.09.018

文献信息

• METHOD FOR DETECTING METHYLATED CYTOSINE BY USING BISULFITE REACTION
  申请人：Wako Pure Chemical Industries, Ltd.

  公开号：EP2799541A1

  公开（公告）日：2014-11-05

  It is an object of the present invention to provide a method for converting non-methylated cytosine in a single-stranded DNA into uracil by a novel bisulfite reaction with higher conversion efficiency from non-methylated cytosine into uracil as compared with the conventional bisulfite method, a method for amplifying the single-stranded DNA in which non-methylated cytosine has been converted into uracil, and a method for detecting methylated cytosine in the single-stranded DNA. The present invention relates to (1) a method for converting non-methylated cytosine in a single-stranded DNA into uracil, comprising subjecting the single-stranded DNA to bisulfite reaction under the presence of at least one of compounds shown by the above-described general formula [1] to [8]; (2) a method for amplifying single-stranded DNA in which non-methylated cytosine has been converted into uracil, comprising further subjecting the single-stranded DNA after bisulfite reaction of (1) to PCR reaction; and (3) a method for detecting methylated cytosine in the aforementioned single-stranded DNA, comprising subjecting the single-stranded DNA amplified in (2) to nucleotide sequence analysis,

  本发明的目的是提供一种通过新型亚硫酸氢盐反应将单链DNA中的非甲基化胞嘧啶转化为尿嘧啶的方法，与传统的亚硫酸氢盐方法相比，该方法将非甲基化胞嘧啶转化为尿嘧啶的效率更高；还提供一种扩增已将非甲基化胞嘧啶转化为尿嘧啶的单链DNA的方法，以及一种检测单链DNA中甲基化胞嘧啶的方法。 本发明涉及（1）一种将单链 DNA 中的非甲基化胞嘧啶转化为尿嘧啶的方法，包括在上述通式[1]至[8]所示化合物中至少一种化合物存在的情况下，将单链 DNA 进行亚硫酸氢盐反应；(2) 扩增非甲基化胞嘧啶已转化为尿嘧啶的单链DNA的方法，包括进一步将(1)亚硫酸氢盐反应后的单链DNA进行PCR反应；以及 (3) 检测上述单链DNA中甲基化胞嘧啶的方法，包括将(2)中扩增的单链DNA进行核苷酸序列分析、