Naphthol as-gr phosphate

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: Naphthol as-gr phosphate
• 英文别名: [3-[(2-methylphenyl)carbamoyl]anthracen-2-yl] dihydrogen phosphate
• 化学式: C₂₂H₁₈NO₅P
• 分子量: 407.362
• InChiKey: AODGGAXCSNLSHI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4
• 重原子数：
  29
• 可旋转键数：
  4
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.045
• 拓扑面积：
  95.9
• 氢给体数：
  3
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  3-羟基蒽-2-羧酸 在 五氯化磷 、 三氯化磷 作用下， 以 1,4-二氧六环 为溶剂， 反应 3.17h， 生成 Naphthol as-gr phosphate
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022

文献信息

• REAGENT SYSTEM AND METHOD
  申请人：Leica Biosystems Melbourne Pty Ltd

  公开号：EP2459983B1

  公开（公告）日：2018-01-17
• IMMUNOASSAY REAGENTS AND METHODS OF USE THEREOF
  申请人：BIOCARE MEDICAL, LLC.

  公开号：US20140057803A1

  公开（公告）日：2014-02-27

  The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and efficiently.
• US5385823A
  申请人：——

  公开号：US5385823A

  公开（公告）日：1995-01-31
• [EN] REAGENT SYSTEM AND METHOD<br/>[FR] SYSTÈME DE RÉACTIF ET PROCÉDÉ
  申请人：LEICA BIOSYSTEMS MELBOURNE PTY

  公开号：WO2011011826A1

  公开（公告）日：2011-02-03

  The present invention relates generally to a chromogenic compositions and reagents systems and methods therefor.