色酚AS-CL磷酸盐 | 18228-16-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 中文名称: 色酚AS-CL磷酸盐
• 中文别名: 萘酚AS-CL-磷酸酯
• 英文名称: 2-Phosphonooxy-naphthalin-3-carbonsaeure-<3-chlor-6-methoxy-anilid>
• 英文别名: naphthol AS-Cl-phosphate；N-(5-chloro-2-methoxyphenyl)-3-(phosphonooxy)naphthalene-2-carboxamide；[3-[(5-chloro-2-methoxyphenyl)carbamoyl]naphthalen-2-yl] dihydrogen phosphate
• CAS: 18228-16-5
• 化学式: C₁₈H₁₅ClNO₆P
• mdl: MFCD00042715
• 分子量: 407.747
• InChiKey: AXMIMEOLDUCZOC-UHFFFAOYSA-N

物化性质

• 密度：
  1.538±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.9
• 重原子数：
  27
• 可旋转键数：
  5
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.055
• 拓扑面积：
  105
• 氢给体数：
  3
• 氢受体数：
  6

安全信息

• 海关编码：
  2924299090

反应信息

• 作为产物：
  描述：

  2-羟基-3-萘甲酸 在 五氯化磷 、 三氯化磷 作用下， 以 1,4-二氧六环 为溶剂， 反应 3.17h， 生成 色酚AS-CL磷酸盐
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022

文献信息

• Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids
  作者：Satoshi. Fujita、Masayoshi. Momiyama、Yasumitsu. Kondo、Naoto. Kagiyama、Samuel H. Hori、Takeshi. Toru

  DOI：10.1021/ac00080a022

  日期：1994.4.15

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.