FREEZE-DRIED PRODUCT FOR TRANSFERRING NUCLEIC ACID, OLIGONUCLEIC ACID OR DERIVATIVE THEREOF
申请人:Koyama, Yoshiyuki
公开号:EP2022853A1
公开(公告)日:2009-02-11
A freeze-dried product is provided which, together with demonstrating satisfactory ability to express function when used to introduce a gene or antisense nucleic acid and the like, enables concentration to be adjusted easily, offers easy handling and has superior storage performance.
A freeze-dried product of a complex containing a nucleic acid, oligonucleic acid or derivative thereof; a cationic polymer, or cationic lipid or aggregate containing the same; and, an anionic polymer; a method for preparing the freeze-dried product, comprising a step of forming a complex by mixing a nucleic acid, oligonucleic acid or derivative thereof; a cationic polymer, or cationic lipid or aggregate containing the same; and an anionic polymer, and a step of freeze-drying the complex; a preparation, reagent or kit containing the freeze-dried product for introducing a nucleic acid, oligonucleic acid or derivative thereof; and, a method for introducing a nucleic acid, oligonucleic acid or derivative thereof into cells that uses the freeze-dried product.
本发明提供了一种冷冻干燥产品,该产品在用于导入基因或反义核酸等时具有令人满意的功能表达能力,而且浓度易于调整,操作简便,储存性能优越。
一种含有核酸、寡核苷酸或其衍生物;阳离子聚合物或阳离子脂质或含有阳离子聚合物或阳离子脂质的聚合体;以及阴离子聚合物的复合物的冻干产品;制备冻干产品的方法,包括通过混合核酸、寡核苷酸或其衍生物形成复合物的步骤;一种制备冻干产品的方法,包括将核酸、寡核苷酸或其衍生物;阳离子聚合物或阳离子脂质或含有阳离子聚合物或阳离子脂质的聚合体;以及阴离子聚合物混合形成复合物的步骤,以及将复合物冻干的步骤;一种含有冻干产品的制剂、试剂或试剂盒,用于导入核酸、寡核苷酸或其衍生物;以及一种使用冻干产品将核酸、寡核苷酸或其衍生物导入细胞的方法。