申请人:Syngenta Participations AG
公开号:EP1574580A2
公开(公告)日:2005-09-14
The invention provides novel methods of controlling gene expression in plastids, using an inducible, transactivator-mediated system, and plants comprising the novel expression systems. The present invention further describes the production of cellulose-degrading enzymes in plants via the application of genetic engineering techniques. Cellulase coding sequences are fused to promoters active in plants and transformed into the nuclear genome and the chloroplast genome. As cellulases may be toxic to plants, preferred promoters are those that are chemically-inducible. In this manner, expression of the cellulase genes transformed into plants may be chemically induced at an appropriate time. In addition, the expressed cellulases may be targeted to vacuoles or other organelles to alleviate toxicity problems. The present invention finds utility in any industrial process requiring a plentiful supply of cellulases, but particularly finds utility in the conversion of cellulosic biomass to ethanol.
本发明提供了利用可诱导的转座子介导系统控制质体中基因表达的新型方法,以及包含新型表达系统的植物。本发明进一步描述了通过应用基因工程技术在植物中生产纤维素降解酶。纤维素酶编码序列与在植物中活跃的启动子融合,并转化到核基因组和叶绿体基因组中。由于纤维素酶可能对植物有毒,因此首选化学诱导型启动子。这样,转化到植物中的纤维素酶基因就可以在适当的时候被化学诱导表达。此外,还可将表达的纤维素酶靶向液泡或其他细胞器,以减轻毒性问题。本发明适用于任何需要大量纤维素酶供应的工业过程,但尤其适用于纤维素生物质转化为乙醇的过程。