Chemiluminescent composition containing cationic surfactants or polymers and 4'hydroxyacetanilide, test kits and their use in analytical methods
申请人:Johnson & Johnson Clinical Diagnostics, Inc.
公开号:EP0582339A2
公开(公告)日:1994-02-09
An aqueous composition useful for providing a chemiluminescent signal in analytical methods is stabilized by the presence of specific amounts of either a low molecular weight cationic surfactant or a cationic polymer. The composition also includes a 2,3-dihydro-1,4-phthalazinedione derivative, such as luminol, and 4'-hydroxyacetanilide. A chemiluminescent signal is generated using the composition in the presence of peroxidase or a peroxidase-labeled specific binding species (such as an antibody or oligonucleotide).
一种用于在分析方法中提供化学发光信号的水性组合物,因含有特定量的低分子量阳离子表面活性剂或阳离子聚合物而变得稳定。该组合物还包括 2,3-二氢-1,4-酞嗪二酮衍生物(如鲁米诺)和 4'-羟基乙酰苯胺。在过氧化物酶或过氧化物酶标记的特异性结合物种(如抗体或寡核苷酸)存在的情况下,使用该组合物会产生化学发光信号。