ASYMMETRIC ONE- AND TWO-PHOTON FLUOROPHORES FOR SIMULTANEOUS DETECTION OF MULTIPLE ANALYTES USING A COMMON EXCITATION SOURCE
申请人:Spangler Brenda D.
公开号:US20090043109A1
公开(公告)日:2009-02-12
A family of one- and two-photon dyes that have overlapping fluorescence excitation maxima between 365 nm and 425 nm and that are specifically functionalized for coupling to biomolecules. The dyes have defined Stokes shifts such that they produce a continuum of emission wavelengths. The dyes are asymmetrical bis stilbenes. The asymmetry of these dyes facilitates functionalization of the dyes with carboxyl, thiol, aldehyde-reactive or amine-reactive groups, which in turn facilitates coupling with macromolecules. Furthermore, the dyes of the present invention are photostable and have high quantum yields (fluorescence intensity).