丹酰氨基-PITC | 102417-94-7

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 中文名称: 丹酰氨基-PITC
• 中文别名: 丹磺酰氨-PITC
• 英文名称: 4-(dansylamino)phenyl isothiocyanate
• 英文别名: 4-(N-1-Dimethylaminonaphthalene-5-sulfonylamino)phenyl isothiocyanate；5-(dimethylamino)-N-(4-isothiocyanatophenyl)naphthalene-1-sulfonamide
• CAS: 102417-94-7
• 化学式: C₁₉H₁₇N₃O₂S₂
• mdl: MFCD00077739
• 分子量: 383.495
• InChiKey: MZGXHCHKGRQLHR-UHFFFAOYSA-N

物化性质

• 熔点：
  125-128 °C
• 溶解度：
  可溶于乙腈、DMSO、甲醇

计算性质

• 辛醇/水分配系数(LogP)：
  5.4
• 重原子数：
  26
• 可旋转键数：
  5
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.105
• 拓扑面积：
  102
• 氢给体数：
  1
• 氢受体数：
  6

安全信息

• 危险品标志：
  Xn
• 安全说明：
  S22,S24/25
• 危险类别码：
  R42

反应信息

• 作为反应物：
  描述：

  6-aminohexyl 2,3,4,6-tetra-O-[3-(α-D-mannopyranosyloxy)propyl]-β-D-glucopyranoside 、 丹酰氨基-PITC 以 N,N-二甲基甲酰胺 为溶剂， 反应 36.0h， 以49%的产率得到6-[4-({[5-(dimethylamino)-1-naphthyl]sulfonyl}amino)phenyl thiourea]-hexyl-2,3,4,6-tetra-O-[3-(α-D-mannopyranosyloxy)-propyl]-β-D-glucopyranoside
  参考文献：
  名称：

  寡糖模拟物的功能化和使用方二酯介导的偶联的多聚化。

  摘要：

  功能化的以碳水化合物为中心的糖簇形成了用于合成标记的寡糖和糖缀合物模拟物的起始材料，其是通过硫脲桥接，肽偶联，尤其是方二酯介导的偶联而获得的。后一种方法也可用于提供新的多价糖缀合物，其在大肠埃希菌细菌的ELISA中经测试其抗黏附特性。

  DOI：

  10.1016/j.carres.2006.12.021

文献信息

• One-Pot Protection-Glycosylation Reactions for Synthesis of Lipid II Analogues
  作者：Katsuhiko Mitachi、Priya Mohan、Shajila Siricilla、Michio Kurosu

  DOI：10.1002/chem.201400307

  日期：2014.4.14

  applied to a one‐pot protection‐glycosylation reaction to form the disaccharide derivative 7 d for the synthesis of lipid II analogues. The temporary protecting group or linker at the C‐6 position and N‐Troc protecting group of 7 d can be cleaved simultaneously through a reductive condition. Overall yields of syntheses of lipid II (1) and neryl‐lipid II Nε‐dansylthiourea are significantly improved by

  (2,6-二氯-4-甲氧基苯基)(2,4-二氯苯基)甲基三氯乙酰亚胺酯( 3 )及其聚合物支撑试剂4可成功应用于一锅保护糖基化反应，形成二糖衍生物7d 用于合成脂质 II 类似物。C-6位的临时保护基或连接基和7d的N -Troc保护基可以通过还原条件同时裂解。通过使用所描述的方法，脂质 II ( 1 ) 和橙花基脂质 II N ε -丹磺基硫脲的合成总产率显着提高。
• Multi-Fluorescent Silica Colloids for Encoding Large Combinatorial Libraries
  作者：Daniel C. Matthews、Lisbeth Grøndahl、Bronwyn J. Battersby、Matt Trau

  DOI：10.1071/ch01120

  日期：——

  Large chemical libraries can be synthesized on solid-support beads by the combinatorial split-and-mix method. A major challenge associated with this type of library synthesis is distinguishing between the beads and their attached compounds. A new method of encoding these solid-support beads, ‘colloidal bar-coding’, involves attaching fluorescent silica colloids (‘reporters’) to the beads as they pass through the compound synthesis, thereby creating a fluorescent bar code on each bead. In order to obtain sufficient reporter varieties to bar code extremely large libraries, many of the reporters must contain multiple fluorescent dyes. We describe here the synthesis and spectroscopic analysis of various mono- and multi-fluorescent silica particles for this purpose. It was found that by increasing the amount of a single dye introduced into the particle reaction mixture, mono-fluorescent silica particles of increasing intensities could be prepared. This increase was highly reproducible and was observed for six different fluorescent dyes. Multi-fluorescent silica particles containing up to six fluorescent dyes were also prepared. The resultant emission intensity of each dye in the multi-fluorescent particles was found to be dependent upon a number of factors; the hydrolysis rate of each silane–dye conjugate, the magnitude of the inherent emission intensity of each dye within the silica matrix, and energy transfer effects between dyes. We show that by varying the relative concentration of each silane–dye conjugate in the synthesis of multi-fluorescent particles, it is possible to change and optimize the resultant emission intensity of each dye to enable viewing in a fluorescence detection instrument. Manuscript received: 13 September 2001. Final version: 18 January 2002.

  大型化学库可以通过组合分裂和混合法在固体支持珠上合成。这种类型的库合成面临的主要挑战是区分珠子和它们附着的化合物。一种新的编码这些固体支持珠的方法，称为“胶体条形码”，涉及将荧光二氧化硅胶体（“报告员”）附着在珠子上，使其在化合物合成过程中形成一个荧光条形码。为了获得足够的报告员品种来对极大的库进行条形码编码，许多报告员必须包含多个荧光染料。我们在这里描述了各种单荧光和多荧光二氧化硅颗粒的合成和光谱分析，以此作为目的。发现通过增加引入反应混合物中的单一染料的量，可以制备出不断增强的单荧光二氧化硅颗粒。这种增加是高度可重复的，并且适用于六种不同的荧光染料。还制备了含有多达六种荧光染料的多荧光二氧化硅颗粒。发现多荧光颗粒中每种染料的发射强度取决于许多因素，包括每个硅烷-染料共轭物的水解速率、每种染料在硅基质内固有的发射强度大小以及染料之间的能量转移效应。我们展示了通过在合成多荧光颗粒时改变每种硅烷-染料共轭物的相对浓度，可以改变和优化每种染料的发射强度，以便在荧光检测仪器中观察。稿件收到日期：2001年9月13日。最终版本：2002年1月18日。
• Method for amino acid sequence analysis
  申请人：SHIMADZU CORPORATION

  公开号：EP0501307A2

  公开（公告）日：1992-09-02

  The present invention is directed to a method for amino acid sequence analysis comprising either the steps of labeling the sample with a fluorescent reagent and quenching the excess fluorescent reagent remaining after said labeling with an ammonium salt, or the steps of degrading amino acid from the amino terminus of peptides or proteins using a fluorescent Edman reagent and quenching the excess fluorescent Edman reagent remaining after said degrading with an ammonium salt. The method of the present invention makes it possible to eliminate the interference of identification by the chromatographic peak of fluorescent reagent by quenching the excess fluorescent reagent in the sample.

  本发明涉及一种氨基酸序列分析方法，包括以下步骤：用荧光试剂标记样品，并用铵盐淬灭标记后剩余的过量荧光试剂；或用荧光埃德曼试剂降解肽或蛋白质氨基末端的氨基酸，并用铵盐淬灭降解后剩余的过量荧光埃德曼试剂。本发明的方法通过淬灭样品中过量的荧光试剂，可以消除荧光试剂色谱峰对鉴定的干扰。
• Apparatus and method for edman degradation using a microfluidic system
  申请人：West Virginia University Research Corporation

  公开号：US20040175822A1

  公开（公告）日：2004-09-09

  An apparatus and method for Edman degradation using a microfluidic system to identify and characterize peptides is disclosed. A microfluidic device comprises an entrance channel through which a substantially purified polypeptide is accepted, a reaction channel engaging the entrance channel wherein the substantially purified polypeptide is digested, producing a digestion product, a reagent reservoir engaging the reaction channel, the reagent reservoir capable of delivering a reagent to the reaction channel, and an exit channel extending from the reaction channel, wherein the digestion product travels through the exit channel upon leaving the reaction channel. Protein digestion on the device comprises delivering a substantially purified polypeptide to a reaction channel, confining the polypeptide in the reaction channel, digesting the polypeptide in the reaction channel producing a digestion product, and removing the digestion product from the reaction channel, wherein the last two steps are repeated until the polypeptide is substantially digested.

  本发明公开了一种利用微流体系统进行埃德曼降解以鉴定和表征多肽的装置和方法。微流控装置包括一个入口通道，通过该入口通道接收基本纯化的多肽；一个与入口通道相接的反应通道，在该反应通道中消化基本纯化的多肽，产生消化产物；一个与反应通道相接的试剂储存器，该试剂储存器能够将试剂输送到反应通道；以及一个从反应通道延伸出来的出口通道，其中消化产物在离开反应通道后通过出口通道。该装置上的蛋白质消化包括将基本纯化的多肽输送到反应通道，将多肽限制在反应通道中，在反应通道中消化多肽产生消化产物，以及从反应通道中移除消化产物，其中重复后两个步骤，直到多肽基本消化为止。
• Apparatus and method for Edman degradation on a microfluidic device utilizing an electroosmotic flow pump
  申请人：Timperman T. Aaron

  公开号：US20050133371A1

  公开（公告）日：2005-06-23

  The present invention comprises an electroosmotic flow pump with both anion and cation exchange beads packed in separate channels that pump towards an intersection. Combining the two flow streams results in higher flowrates for the pump and allows operation of the pump over a wide pH range. The pump can be used to deliver solutions ranging from a pH of about 2 to about 12. In a preferred embodiment, the electroosmotic pump of the present invention is fabricated on a microfluidic device capable of Edman degradation. In a preferred embodiment of the present invention, the beads are immobilized in the channels using weirs and membranes, eliminating the need for frits. The pump may be comprised of capillaries. Additionally, the electroosmotic flow pump of the present invention may be integrated into an Integrated Microfluidic Proteome Analysis System.

  本发明包括一种电渗流泵，阴离子和阳离子交换珠分别装在不同的通道中，向一个交叉点泵送。将两股流结合在一起可提高泵的流速，使泵在较宽的 pH 值范围内运行。该泵可用于输送 pH 值从约 2 到约 12 的溶液。在一个优选的实施方案中，本发明的电渗泵是在能够进行埃德曼降解的微流体装置上制造的。在本发明的一个优选实施方案中，使用堰和膜将珠子固定在通道中，无需使用熔块。泵可由毛细管组成。此外，本发明的电渗流泵可集成到集成微流体蛋白质组分析系统中。

表征谱图