申请人:KIKKOMAN CORPORATION
公开号:EP1291416A1
公开(公告)日:2003-03-12
The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase.
A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.
本发明的目的是提供一种新型果糖基肽氧化酶,它具有优异的理化性质,如稳定性,可作为一种酶用于临床诊断,本发明的目的还包括提供一种生产果糖基肽氧化酶的方法。
本文提供了一种新型果糖基肽氧化酶,其理化性质可用作临床诊断用酶,还提供了一种生产新型果糖基肽氧化酶的方法,该方法包括:在培养基中培养能够产生氧化酶的微生物;从培养物中收集氧化酶。此外,本文还提供了编码新型果糖基肽氧化酶的果糖基肽氧化酶基因、将该基因插入载体 DNA 的重组 DNA 以及生产新型果糖基肽氧化酶的方法,该方法包括:在培养基中培养包括该基因的转化体或转导体;以及从培养物中收集新型果糖基肽氧化酶。