5-ROX-CF

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 5-ROX-CF
• 英文别名: 4-[4-[[[3-Carboxy-4-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoyl]amino]methyl]-3-hydroxy-6-oxoxanthen-9-yl]benzene-1,3-dicarboxylic acid
• 化学式: C₅₅H₄₄N₃O₁₁
• 分子量: 922.968
• InChiKey: YYVVBHHTTDDXRS-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  5.6
• 重原子数：
  69
• 可旋转键数：
  8
• 环数：
  12.0
• sp3杂化的碳原子比例：
  0.24
• 拓扑面积：
  203
• 氢给体数：
  5
• 氢受体数：
  12

反应信息

• 作为产物：
  描述：

  4'-aminomethyl-5-carboxyfluorescein 、 5-ROX-NHS 在 三乙胺 作用下， 以 二甲基亚砜 、 乙腈 为溶剂， 生成 5-ROX-CF
  参考文献：
  名称：

  Method for detecting oligonucleotides using energy transfer dyes with long stoke shift

  摘要：

  提供了一种新的连接剂，用于将给体染料与受体染料连接在能量转移荧光染料中。这些连接剂促进了给体染料和受体染料之间的能量高效转移。其中一种连接剂用于将给体染料与受体染料连接在能量转移荧光染料中，具有一般结构R21Z1C（O）R22R26，其中R21是附加在给体染料上的C1-5烷基，C（O）是一个羰基，Z1是NH、硫或氧，R22是一个取代基，包括烯烃、二烯、炔烃、至少有一个不饱和键的五元和六元环或附加在羰基碳上的融合环结构，而R28包括一个功能基团，将连接剂连接到受体染料上。

  公开号：

  US06335440B1

文献信息

• PHOSPHONYLATED FLUORESCENT DYES AND CONJUGATES
  申请人：Lukhtanov Eugeny

  公开号：US20080207887A1

  公开（公告）日：2008-08-28

  Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.

  试剂提供了将膦酸酯基团引入荧光染料中的方法。同时还提供了制备染料偶联物的方法。
• Phosphonate fluorescent dyes and conjugates
  申请人：Lukhtanov Eugeny

  公开号：US20070172832A1

  公开（公告）日：2007-07-26

  Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.

  提供试剂用于将膦酸基团引入荧光染料中。同时还提供了制备染料共轭物的方法。
• PHOSPHONATE FLUORESCENT DYES AND CONJUGATES
  申请人：Lukhtanov Eugeny

  公开号：US20100261903A1

  公开（公告）日：2010-10-14

  Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.

  试剂提供了将膦酸酯基团引入荧光染料中的方法。还提供了制备染料偶联物的方法。
• METHODS OF ANALYZING POLYNUCLEOTIDES EMPLOYING ENERGY TRANSFER DYES
  申请人：Lee G. Linda

  公开号：US20070154925A1

  公开（公告）日：2007-07-05

  Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R 21 Z 1 C(O)R 22 R 28 where R 21 is a C 1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z 1 is either NH, sulfur or oxygen, R 22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R 28 includes a functional group which attaches the linker to the acceptor dye.
• ENERGY TRANSFER DYES WITH ENHANCED FLUORESCENCE
  申请人：Lee G. Linda

  公开号：US20070154927A1

  公开（公告）日：2007-07-05

  Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R 21 Z 1 C(O)R 22 R 28 where R 21 is a C 1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z 1 is either NH, sulfur or oxygen, R 22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R 28 includes a functional group which attaches the linker to the acceptor dye.