2-(4-吗啉基)-8-苯基-4H-1-苯并吡喃-4-酮盐酸盐 | 934389-88-5

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 2-(4-吗啉基)-8-苯基-4H-1-苯并吡喃-4-酮盐酸盐
• 英文名称: LY-294,002 hydrochloride
• 英文别名: 2-morpholin-4-yl-8-phenylchromen-4-one;hydrochloride
• CAS: 934389-88-5
• 化学式: C19H18ClNO3
• 分子量: 343.8
• InChiKey: OQZQSRICUOWBLW-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.72
• 重原子数：
  24
• 可旋转键数：
  2
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.21
• 拓扑面积：
  38.8
• 氢给体数：
  1
• 氢受体数：
  4

文献信息

• Method for culture of stem cell
  申请人：Sasai Yoshiki

  公开号：US10227563B2

  公开（公告）日：2019-03-12

  The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming homogenous aggregates of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as suspended aggregates in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.

  本发明通过快速形成干细胞均质聚集体的步骤，实现了干细胞在无血清培养基中的高效悬浮培养，并提供了一种选择性诱导干细胞分化神经的方法、一种在体外形成大脑皮层神经网络的方法和一种在体外产生脑组织立体结构的方法、以及一种生产下丘脑神经元祖细胞的方法，包括在基本上不含 Nodal 信号启动子、Wnt 信号启动子、FGF 信号启动子、BMP 信号启动子、维甲酸和胰岛素的无血清培养基中培养悬浮聚集的多能干细胞，并从培养物中分离出下丘脑神经元祖细胞。
• Producing mesodermal cell types and methods of using the same
  申请人：The Board of Trustees of the Leland Stanford Junior University

  公开号：US10787640B2

  公开（公告）日：2020-09-29

  Methods are provided for the generation of mesodermal cell types and derivatives thereof. Also provided are methods for generating purified populations of mesodermal cell types and derivatives thereof. The instant disclosure also provides methods of screening for cellular responses of the generated mesodermal cell types and derivatives thereof. Also provide are methods for screening for organismal phenotypes induced by introduction of the generated mesodermal cell types and derivatives thereof. Treatment methods making use of the generated mesodermal cell types and derivatives thereof are also provided. The instant disclosure also provides systems, compositions, and kits for practicing the methods of the disclosure.

  提供了生成中胚层细胞类型及其衍生物的方法。还提供了生成纯化的中胚层细胞类型及其衍生物的方法。本发明还提供了筛选所产生的中胚层细胞类型及其衍生物的细胞反应的方法。本发明还提供了通过引入所产生的中胚层细胞类型及其衍生物来筛选生物表型的方法。还提供了利用生成的中胚层细胞类型及其衍生物的治疗方法。本发明还提供了用于实施本发明方法的系统、组合物和试剂盒。
• Method for culturing stem cell
  申请人：RIKEN

  公开号：US10808224B2

  公开（公告）日：2020-10-20

  The present invention provides a method of obtaining aggregates containing a rostral hypothalamus tissue and a rostral head ectodermal tissue, a hypophysis precursor tissue and a hypophysis hormone producing cell, by using a serum-free medium (preferably substantially free of growth factor and insulins), forming homogeneous aggregates of stem cells from pluripotent stem cells such as ES cell and the like, which are plated at a high cell concentration, and subjecting the formed aggregates to floating-culture.

  本发明提供了一种获得含有喙下丘脑组织和喙头外胚层组织、下胚乳前体组织和下胚乳激素产生细胞的聚集体的方法，该方法通过使用无血清培养基（最好基本上不含生长因子和胰岛素），将多能干细胞（如ES细胞等）形成均匀的干细胞聚集体，并将其进行高细胞浓度的培养，然后将形成的聚集体进行漂浮培养。
• METHOD FOR CULTURING STEM CELL
  申请人：RIKEN

  公开号：EP2774983B1

  公开（公告）日：2019-05-15
• EXPANSION AND DIFFERENTIATION OF INNER EAR SUPPORTING CELLS AND METHODS OF USE THEREOF
  申请人：Massachusetts Eye & Ear Infirmary

  公开号：EP3407901B1

  公开（公告）日：2021-07-21