溴化二甲基(2-羟基-5-硝基苯甲基)锍 | 28611-73-6

• 分子结构分类: 有机化合物 - 苯类化合物 - 酚类
• 中文名称: 溴化二甲基(2-羟基-5-硝基苯甲基)锍
• 中文别名: 二甲基(2-羟基-5-硝基苄基)溴化锍
• 英文名称: Dimethyl(2-hydroxy-5-nitrobenzyl)sulfonium bromide
• 英文别名: (2-hydroxy-5-nitrophenyl)methyl-dimethylsulfanium;bromide
• CAS: 28611-73-6
• 化学式: C9H12BrNO3S
• 分子量: 294.17
• InChiKey: VEGVMTIJKACZGO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.32
• 重原子数：
  15
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  67
• 氢给体数：
  1
• 氢受体数：
  4

安全信息

• 危险品标志：
  Xi
• 危险类别码：
  R36/37/38
• 安全说明：
  S26,S36
• WGK Germany：
  3

文献信息

• Isolation, purification, characterization, cloning and sequencing of N-alpha-acetyltransferase
  申请人：THE GENERAL HOSPITAL CORPORATION

  公开号：EP0334004A1

  公开（公告）日：1989-09-27

  There was isolated and purified a Nα-acetyltransferase with a molecular weight of about 180,000 daltons which is composed of two subunit peptides having molecular weights of about 95,000 each, having high enzyme activity. The gene coding for this enzyme was isolated and sequenced. It can be incorporated into recombinant DNA molecules and expression vehicles for transforming cells, especially plant cells. These cells can be cultivated to plants having an enhanced acetylation capability.

  分离并纯化了一种分子量约为 180,000 道尔顿的 Nα 乙酰转移酶，它由两个分子量各约为 95,000 道尔顿的亚基肽组成，具有很高的酶活性。这种酶的编码基因已被分离和测序。它可被纳入重组 DNA 分子和表达载体，用于转化细胞，特别是植物细胞。这些细胞可以培养成具有更强乙酰化能力的植物。
• Purification and characterization of an acetyl-CoA hydrolase
  申请人：THE GENERAL HOSPITAL CORPORATION

  公开号：EP0348958A2

  公开（公告）日：1990-01-03

  This invention is directed to the isolation, identification, purification and characterization of acetyl-CoA hydrolase, the enzyme having a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity wherein 1 unit of acetyltransferase activity is defined as the amount of enzyme able to transfer 1 pmol of [³H] acetyl group from [³H] acetyl coenzyme A to ACTH (1-24) by acetyltransferase standard enzyme assay under standard conditions. This invention is further directed to a method for purifying the acetyl-CoA hydrolase.

  本发明涉及乙酰-CoA水解酶的分离、鉴定、纯化和表征，该酶的分子量约为64、1 单位乙酰辅酶水解酶活性是指能够抑制 1 单位乙酰转移酶活性的酶量，其中 1 单位乙酰转移酶活性是指在标准条件下，通过乙酰转移酶标准酶测定，能够将 1 pmol 的[³H]乙酰基从[³H]乙酰辅酶 A 转移到 ACTH（1-24）的酶量。本发明还涉及一种纯化乙酰-CoA水解酶的方法。
• Recombinant DNA sequences encoding feedback inhibition released enzymes, plasmids comprising the recombinant DNA sequences, transformed microorganisms useful in the production of aromatic amino acids, and a process for preparing aromatic amino acids by fermentation
  申请人：Ajinomoto Co., Inc.

  公开号：EP0488424A2

  公开（公告）日：1992-06-03

  A method for releasing feedback inhibition of the key enzymes in the production of aromatic amino acids by fermentation is disclosed. Aromatic amino acids are prepared by a process which comprises transforming a microorganism with a recombinant DNA sequence bearing a gene or gene group encoding a feedback inhibition-released enzyme in the phenylalanine and/or tryptophan biosynthetic pathway, obtained by substituting one or two amino acid residue(s) or deleting one or more amino acid residue(s) of 3-deoxy-D-arabinoheptulonic acid 7-phosphate synthase (DS) or prephenate dehydratase, culturing the microorganism and isolating the aromatic amino acid produced in the medium. Higher efficiency and improved yields in the production of L-phenylalanine and L-tryptophan by fermentation are realized.

  本发明公开了一种释放发酵法生产芳香族氨基酸过程中关键酶的反馈抑制的方法。芳香族氨基酸的制备过程包括用带有编码苯丙氨酸和/或色氨酸生物合成途径中反馈抑制释放酶的基因或基因组的重组 DNA 序列转化微生物、通过替换 3-脱氧-D-阿拉伯庚二酸 7-磷酸合成酶（DS）或苯丙氨酸脱水酶的一个或两个氨基酸残基或删除一个或多个氨基酸残基，培养微生物并分离培养基中产生的芳香族氨基酸而获得。通过发酵生产 L-苯丙氨酸和 L-色氨酸的效率更高，产量更大。
• The glucose-regulated promoter of yeast acetyl-CoA hydrolase
  申请人：THE GENERAL HOSPITAL CORPORATION

  公开号：EP0584536A1

  公开（公告）日：1994-03-02

  This invention is directed to the acetyl-CoA hydrolase enzyme of yeast and to a method for purifying acetyl-CoA hydrolase. The enzyme has a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 units wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity and 1 unit of acetyltransferase activity is defined as the amount of enzyme needed to transfer 1 pmol of [³H] acetyl group from [³H] acetyl coenzyme A to ACTH (1-24) in the acetyltransferase standard enzyme assay under standard conditions. The invention is further directed to the glucose-repressible promoter which regulates the expression of the acetyl-CoA hydrolase gene in yeast, to vectors incorporating this promoter and to host cells transformed with such vectors.

  本发明涉及酵母的乙酰-CoA水解酶和纯化乙酰-CoA水解酶的方法。该酶的分子量约为 64、000道尔顿，酶活性大于20单位，其中1单位乙酰CoA水解酶活性定义为能够抑制1单位乙酰转移酶活性的酶量，1单位乙酰转移酶活性定义为在标准条件下乙酰转移酶标准酶测定中将1pmol[³H]乙酰基从[³H]乙酰辅酶A转移到ACTH（1-24）所需的酶量。本发明还涉及调节酵母中乙酰-CoA水解酶基因表达的葡萄糖可抑制启动子、含有这种启动子的载体以及用这种载体转化的宿主细胞。
• [EN] IDENTIFICATION OF METHIONINE N-ALPHA-ACETYLTRANSFERASE
  申请人：THE GENERAL HOSPITAL CORPORATION

  公开号：WO1991006636A1

  公开（公告）日：1991-05-16

  (EN) A Methionine N$g(a)-acetyltransferase enzyme has been identified in yeast. The existence of the enzyme defines a gene for methionine N$g(a)-acetyltransferase which may be cloned and altered. Cells expressing the normal or variant forms of this enzyme are valuable tools for determining the amino acid sequence of uncharacterized proteins. Such cells are also valuable tools for expressing a recombinant protein lacking an acetyl group at its $g(a)-amino group.(FR) Une enzyme de methionine N$g(a)-acétyltransférase a été identifiée dans la levure. L'existence de l'enzyme définit un gène pour la méthionine N$g(a)-acétyltransférase qui peut être clonée et altérée. Des cellules exprimant les formes normales ou les variantes de forme de cette enzyme sont des outils de valeur pour déterminer la séquence d'acides aminés de protéines non caractérisées. De telles cellules sont également des outils efficaces pour exprimer une protéine de recombinaison manquant d'un groupe acétyl au niveau de son groupe $g(a)-amino.

  以下为英文文本的中文翻译： (EN) 一种名为甲硫氨酸 N$g(a)-甲基转移酶的酶在酵母中被识别出来。这种酶的存在定义了一个关于甲硫氨酸 N$g(a)-甲基转移酶的基因，该基因可以通过克隆和修改技术进行拷贝。表达正常或变异形式的这种酶的细胞是非常有用的工具，用于确定未明化的蛋白质的氨基酸序列。它们也是成功表达一个缺乏 $g(a)-氨基甲基的重组蛋白的有用工具。(FR) 一种名为甲硫氨酸 N$g(a)-甲基转移酶的酶被酵母中发现。这种酶的存在定义了一个关于甲硫氨酸 N$g(a)-甲基转移酶的基因，该基因可以被克隆并修改。表达正常或变异形式的该酶的细胞是非常有用的工具，用于确定未明化的蛋白质的氨基酸序列。它们也是一种成功的工具来表达一个缺少 $g(a)-氨基甲基的重组蛋白。