(22E,24xi)-Stigmasta-5,22-dien-3-ol | 72903-53-8

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 类固醇和类固醇衍生物
• 英文名称: (22E,24xi)-Stigmasta-5,22-dien-3-ol
• 英文别名: (8S,9S,10R,13R,14S,17R)-17-[(E,2R)-5-ethyl-6-methylhept-3-en-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol
• CAS: 72903-53-8
• 化学式: C₂₉H₄₈O
• 分子量: 412.7
• InChiKey: HCXVJBMSMIARIN-JBNNPUILSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  8.6
• 重原子数：
  30
• 可旋转键数：
  5
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.86
• 拓扑面积：
  20.2
• 氢给体数：
  1
• 氢受体数：
  1

文献信息

• Compositions and method for stable isotope labelling of biological compounds
  申请人：Egorova-Zachernyuk A. Tatiana

  公开号：US20070082399A1

  公开（公告）日：2007-04-12

  The present invention is concerned the labelling of biological compounds with stable isotopes such that the three-dimensional structure of the biological compounds may be analysed by e.g. NMR spectroscopy. The invention employs microorganisms that are grown on mineral media comprising carbon and nitrogen sources that contain stable isotopes to produce biomass that is uniformly labelled with stable isotopes. The biomass may be autolysed to produce an autolysate. The biomass may further be extracted with organic solvent to produce lipids. The (delipidised) biomass is hydrolysed to produce labelled amino acids and other nutrients, which are used together with the autolysate, extracted lipids and further components to compose a culture medium for a mammalian or insect host cells for the production of biological compounds that are uniformly labelled with stable isotopes. The biological compound preferably is a biological acromolecule, such as e.g. a mammalian membrane protein. Data supplied from the esp@cenet database—Worldwide

  本发明涉及将生物化合物用稳定同位素标记，以便通过例如NMR光谱分析生物化合物的三维结构。本发明采用在含有含稳定同位素的碳和氮源的矿物质培养基上生长的微生物，以产生均匀标记有稳定同位素的生物量。生物量可以自溶产生自溶液。生物量还可以用有机溶剂提取产生脂质。去除脂质的生物量被水解以产生标记的氨基酸和其他营养物质，这些营养物质与自溶液、提取的脂质和进一步的成分一起用于组成哺乳动物或昆虫宿主细胞的培养基，以生产均匀标记有稳定同位素的生物化合物。生物化合物最好是生物大分子，例如哺乳动物膜蛋白。数据来源于esp@cenet数据库-全球。
• PHOSPHOCHOLINE SURFACTANTS AND THEIR USE
  申请人：SuperGen, Inc.

  公开号：EP1150685A1

  公开（公告）日：2001-11-07
• EP1150685A4
  申请人：——

  公开号：EP1150685A4

  公开（公告）日：2005-06-15
• COMPOSITIONS AND METHODS FOR STABLE ISOTOPE LABELLING OF BIOLOGICAL COMPOUNDS
  申请人：EGOROVA-ZACHERNYUK, Tatiana A.

  公开号：EP1644474A2

  公开（公告）日：2006-04-12
• Compositions and methods for stable isotope labelling of biological compounds
  申请人：Egorova-Zachernyuk Tatiana A.

  公开号：US20210047607A1

  公开（公告）日：2021-02-18

  The present invention is concerned the labelling of biological compounds with stable isotopes such that the three-dimensional structure of the biological compounds may be analysed by e.g. NMR spectroscopy. The invention employs microorganisms that are grown on mineral media comprising carbon and nitrogen sources that contain stable isotopes to produce biomass that is uniformly labelled with stable isotopes. The biomass may be autolysed to produce an autolysate. The biomass may further be extracted with organic solvent to produce lipids. The (delipidised) biomass is hydrolysed to produce labelled amino acids and other nutrients, which are used together with the autolysate, extracted lipids and further components to compose a culture medium for a mammalian or insect host cells for the production of biological compounds that are uniformly labelled with stable isotopes. The biological compound preferably is a biological macromolecule, such as e.g. a mammalian membrane protein.