| 881178-42-3

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• CAS: 881178-42-3
• 化学式: C₃₅H₃₄N₃O₇
• 分子量: 608.671
• InChiKey: UFKKZQWRQUJSEX-UHFFFAOYSA-O

反应信息

• 作为反应物：
  描述：

  [[(2R,3S,5R)-3-[(E)-7-aminohept-2-enoxy]-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate 、 以 aq. phosphate buffer 、 二甲基亚砜 为溶剂， 生成
  参考文献：
  名称：

  Synthesis of 3′-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis

  摘要：

  Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2013.11.040

文献信息

• Immuno‐Desorption Electrospray Ionization Mass Spectrometry Imaging Identifies Functional Macromolecules by Using Microdroplet‐Cleavable Mass Tags
  作者：Xiaowei Song、Qingce Zang、Chao Li、Tianhao Zhou、Richard N. Zare

  DOI：10.1002/anie.202216969

  日期：2023.2.20

  Water-microdroplet-cleavable, bifunctional boronate mass tags are introduced to conduct cell-resolved immunofluorescence imaging followed by multiplex immuno-desorption electrospray ionization mass spectrometry imaging.

  引入水微滴可裂解双功能硼酸盐质量标签进行细胞分辨免疫荧光成像，然后进行多重免疫解吸电喷雾电离质谱成像。