The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.
本发明涉及 RNA 扩增方法,包括使用恒温
反转录酶从 RNA 反转录 cDNA 的方法。在一个特定方面,这些方法能够通过多个 cDNA 合成周期对 RNA 模板进行线性扩增。