A new and improved method and composition for determining the presence and concentration of low to trace amounts of proteins, such as albumin, in a test sample, such as urine. The method includes using a reagent composition capable of interacting with low to trace amounts of proteins to produce a visually or instrumentally detectable and/or measurable response. The method can be used in wet assays or in dry test strip assays, wherein the reagent composition is incorporating into a carrier matrix. The new and improved reagent composition, comprising a dye, such as a polyhydroxybenzenesulfonephthalein-type indicator, like pyrocatechol violet; a tungstate, such as ammonium tungstate; and, if necessary, a suitable buffer, is incorporated into the carrier matrix to provide sufficient sensitivity to low to trace protein levels and sufficient color resolution between low to trace protein levels, thereby affording an accurate and trustworthy protein assay of test samples having a low protein concentration.
一种新的和改进的方法及组合物,用于测定检测样品(如尿液)中低至痕量的蛋白质(如白蛋白)的存在和浓度。该方法包括使用一种试剂组合物,该试剂组合物能够与低量至痕量的蛋白质相互作用,产生视觉上或仪器上可检测和/或测量的反应。该方法可用于湿法检测或干法试纸检测,其中试剂组合物与载体基质结合。新的改进型试剂组合物包括
染料,如多羟基苯磺
酞类指示剂,如焦
儿茶酚紫;
钨酸盐,如
钨酸
铵;必要时还包括适当的缓冲液,将其加入载体基质中,可提供对低至痕量蛋白质
水平的足够灵敏度,以及低至痕量蛋白质
水平之间的足够颜色分辨率,从而对具有低蛋白质浓度的测试样品进行准确、可靠的蛋白质检测。