3-prop-1-ynyl-4,6-diaminopyrazolo[3,4-d]pyrimidine | 637338-75-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡唑并嘧啶类
• 英文名称: 3-prop-1-ynyl-4,6-diaminopyrazolo[3,4-d]pyrimidine
• 英文别名: 3-prop-1-ynyl-2H-pyrazolo[3,4-d]pyrimidine-4,6-diamine
• CAS: 637338-75-1
• 化学式: C₈H₈N₆
• 分子量: 188.192
• InChiKey: CCOZPJBSNPRNTK-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.3
• 重原子数：
  14
• 可旋转键数：
  1
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.12
• 拓扑面积：
  107
• 氢给体数：
  3
• 氢受体数：
  5

文献信息

• Fluorescent probes for DNA detection by hybridization with improved sensitivity and low background
  申请人：Lokhov Sergey

  公开号：US20050214797A1

  公开（公告）日：2005-09-29

  Minor groove binder-oligonucleotide probes are provided along with methods for their use wherein the probes have an attached fluorophore which, in an unhybridized form exhibits very low background signal.

  提供了与其使用方法一起的小沟结合物-寡核苷酸探针，其中这些探针带有一个附加的荧光物质，未杂交形式下显示出非常低的背景信号。
• FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
  申请人：Vorobiev Alexei

  公开号：US20130261014A1

  公开（公告）日：2013-10-03

  3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

  提供了3-炔基肌苷类似物及其作为通用碱基的用途。这些肌苷类似物可以被合并到核酸引物和探针中。它们不会显著破坏核酸双链。因此，包含这些肌苷类似物的新型核酸引物和探针可以用于各种方法。这些类似物意外地作为通用碱基发挥良好作用。它们不仅比嘌呤对A、C、T和G稳定双链更多，而且在引物中被聚合酶识别，从而实现高效扩增。
• Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis
  申请人：Epoch Biosciences, Inc.

  公开号：US20030175728A1

  公开（公告）日：2003-09-18

  Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.

  提供了寡核苷酸探针/共轭物，并提供了它们在检测扩增过程中的使用方法，其中产生的信号不依赖于5'核酸酶消化。
• FLUORESCENT PROBES FOR DNA DETECTION BY HYBRIDIZATION WITH IMPROVED SENSITIVITY AND LOW BACKGROUND
  申请人：Lokhov Sergey

  公开号：US20080293586A1

  公开（公告）日：2008-11-27

  Minor groove binder-oligonucleotide probes are provided along with methods for their use wherein the probes have an attached fluorophore which, in an unhybridized form exhibits very low background signal.

  提供了微小沟结合剂寡核苷酸探针以及其使用方法，其中探针具有附加的荧光物质，未杂交形式的荧光物质信号非常低。
• Methods and compositions for detecting antibiotic resistant bacteria
  申请人：ELITechGroup B.V.

  公开号：US10266903B2

  公开（公告）日：2019-04-23

  Primers and probes specific to the genes encoding extended spectrum beta-lactamase that involves CTX-M groups 1 and 9 that cause extended beta-lactamase resistance in bacteria are described herein, with methods and kits for using these primers and probes to detect CTX-M groups 1 and 9 nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the CTX-M groups 1 and 9 gene are amplified and corresponding sequences for CTX-M groups 1 and 9 are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.

  本文描述了编码涉及 CTX-M 组 1 和 9 的扩谱 beta-内酰胺酶基因的特异性引物和探针，以及使用这些引物和探针检测 CTX-M 组 1 和 9 核酸的方法和试剂盒。在所述方法中，对从怀疑含有 CTX-M 1 和 9 族基因的生物样本或组织中获得的临床或检测样本中的核酸进行扩增，并检测 CTX-M 1 和 9 族的相应序列。扩增的核酸可通过各种最新技术方法进行检测，包括荧光共振能量转移（FRET）、放射性标记、酶标记等。