fluorescein-5EX-NHS | 1196157-68-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: fluorescein-5EX-NHS
• 英文别名: fluorescein-5-EX succinimidyl ester；Fluorescein-5-EX N-hydroxysuccinimide ester；5-[[2-[3-(2,5-dioxopyrrolidin-1-yl)oxy-3-oxopropyl]sulfanylacetyl]amino]-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
• CAS: 1196157-68-2
• 化学式: C₂₉H₂₂N₂O₁₀S
• 分子量: 590.567
• InChiKey: CBYGNGZRFGVXDG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.2
• 重原子数：
  42
• 可旋转键数：
  10
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.17
• 拓扑面积：
  202
• 氢给体数：
  3
• 氢受体数：
  11

反应信息

• 作为反应物：
  描述：

  fluorescein-5EX-NHS 、 N-(4-(((2-amino-9H-purin-6-yl)oxy)methyl)benzyl)-4-(aminomethyl)benzamide 在 N,N-二异丙基乙胺 作用下， 以 二甲基亚砜 为溶剂， 反应 1.0h， 以40%的产率得到5-[[2-[3-[[4-[[4-[(2-amino-7H-purin-6-yl)oxymethyl]phenyl]methylcarbamoyl]phenyl]methylamino]-3-oxopropyl]sulfanylacetyl]amino]-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
  参考文献：
  名称：

  Substrates of O6-alkylguanine-DNA alkyltransferase and mutants thereof

  摘要：

  该发明涉及公式（I′）的化合物：其中A、L2、M和B如描述中所定义。这些化合物是O6-烷基鸟嘌呤-DNA烷基转移酶及其突变体的底物。

  公开号：

  US09024022B2

文献信息

• Discovery and Development of Small-Molecule Inhibitors of Glycogen Synthase
  作者：Buyun Tang、Mykhaylo S. Frasinyuk、Vimbai M. Chikwana、Krishna K. Mahalingan、Cynthia A. Morgan、Dyann M. Segvich、Svitlana P. Bondarenko、Galyna P. Mrug、Przemyslaw Wyrebek、David S. Watt、Anna A. DePaoli-Roach、Peter J. Roach、Thomas D. Hurley

  DOI：10.1021/acs.jmedchem.9b01851

  日期：2020.4.9

  at 2.85 Å, as well as kinetic data, revealed that H23 bound within the uridine diphosphate glucose binding pocket of yGsy2p. The high conservation of residues between human and yeast GS in direct contact with H23 informed the development of around 500 H23 analogs. These analogs produced a structure-activity relationship profile that led to the identification of a substituted pyrazole, 4-(4-(4-hydro

  糖原的过度积累是各种糖原贮积病（GSD）的标志，包括庞贝病，科里病，安徒生病和拉福拉病。越来越多的证据表明，抑制糖原累积代表了治疗这些GSD的潜在治疗方法。使用设计用于筛选关键糖原合成酶，糖原合成酶（GS）抑制剂的荧光偏振分析，我们确定了取代的咪唑（rac）-2-甲氧基-4-（1-（2-（1-（1-甲基吡咯烷酮- （2-基）乙基）-4-苯基-1H-咪唑-5-基）苯酚（H23），是酵母GS 2（yGsy2p）的首批抑制剂。来自X射线晶体学在2.85Å处的数据以及动力学数据表明，H23结合在yGsy2p的尿苷二磷酸葡萄糖结合口袋中。直接与H23接触的人与酵母GS之间残基的高度保守性有助于开发约500种H23类似物。这些类似物产生了结构-活性关系图，从而鉴定了取代的吡唑4-（4-（4-羟苯基）-3-（三氟甲基）-1H-吡唑-5-基）邻苯三酚和300-对人GS的效力提高了两倍。这些取代的吡唑具有有
• Substantially cell membrane impermeable compound and use thereof
  申请人：New South Innovations PTY Limited

  公开号：US07074766B1

  公开（公告）日：2006-07-11

  The present invention relates to a compound according to Formula (I): A-(L-Y)p, wherein A comprises at least one substantially cell-membrane impermeable pendant group; L comprises any suitable linker and/or spacer group; Y comprises at least one arsenoxide or arsenoxide equivalent; p is an integer from 1 to 10; and the sum total of carbon atoms in A and L together, is greater than 6.

  本发明涉及一种化合物，其符合以下式（I）：A-(L-Y)p，其中A包括至少一个基本上细胞膜不透性的吊挂基团；L包括任何合适的连接基团和/或间隔基团；Y包括至少一个砷酸盐或砷酸盐等效物；p为1至10的整数；A和L中碳原子的总和大于6。
• METHODS AND COMPOSITIONS FOR ANALYZING PROTEINS
  申请人：Singh Sharat

  公开号：US20080311674A1

  公开（公告）日：2008-12-18

  Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide. The interaction between the first binding agent and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.

  本文公开了一种用于确定样品中经历了翻译后修饰的一个或多个目标多肽的方法、组合物和试剂盒。将样品和第一试剂混合，第一试剂包括一个裂解诱导基团和一个针对目标多肽上的结合位点的第一结合剂，然后将其置于相应的结合基团发生结合的条件下。结合位点是涉及目标多肽的翻译后修饰活性的结果。该方法可用于确定目标多肽本身。在另一实施例中，目标多肽的存在和/或数量与参与目标多肽翻译后修饰的酶等活性剂的存在和/或数量及/或活性相关。第一结合剂与结合位点之间的相互作用将裂解诱导基团带到可裂解基团附近，可裂解基团与多肽相关，并仅在接近裂解诱导基团时易受到裂解。通过这种方式，每个多肽的电泳标记可以被释放。释放的电泳标记被分离，并根据相应的电泳标记确定目标多肽的存在和/或数量。
• Methods and Compositions for Analyzing Proteins
  申请人：Singh Sharat

  公开号：US20120088307A1

  公开（公告）日：2012-04-12

  Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification.

  本发明公开了用于确定样品中已经经历了翻译后修饰的一个或多个目标多肽的方法、组合物和试剂盒。
• NOVEL SUBSTRATES OF O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE AND MUTANTS THEREOF
  申请人：Bourrier Emmanuel

  公开号：US20110236952A1

  公开（公告）日：2011-09-29

  The invention relates to compounds of formula (I′): in which A, L 2 , M and B are as defined in the description. These compounds are substrates of O 6 -alkylguanine-DNA alkyltransferase and mutants

  本发明涉及式(I')的化合物： 其中，A，L2，M和B如描述中所定义。这些化合物是O6-烷基鸟嘌呤-DNA烷基转移酶和突变体的底物。