4a-Formyl-5a-cholesta-8,24-dien-3b-ol

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 类固醇和类固醇衍生物
• 英文名称: 4a-Formyl-5a-cholesta-8,24-dien-3b-ol
• 英文别名: 3-hydroxy-10,13-dimethyl-17-(6-methylhept-5-en-2-yl)-2,3,4,5,6,7,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-4-carbaldehyde
• 化学式: C₂₈H₄₄O₂
• 分子量: 412.6
• InChiKey: ZLQSSFNCEUGGJF-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  7.2
• 重原子数：
  30
• 可旋转键数：
  5
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.82
• 拓扑面积：
  37.3
• 氢给体数：
  1
• 氢受体数：
  2

文献信息

• COMPOSITIONS AND METHODS FOR METABOLIC SELECTION OF TRANSFECTED CELLS
  申请人：Biofactura, Inc

  公开号：EP1910421B1

  公开（公告）日：2019-03-13
• Compositions and Methods for Metabolic Selection of Transfected Cells
  申请人：Branco Luis

  公开号：US20100028940A1

  公开（公告）日：2010-02-04

  The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol/cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and/or producing the heterologous protein in these cells in chemically defined and/or serum-free media.