pyocyanin | 392287-05-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二氮杂萘
• 英文名称: pyocyanin
• 英文别名: pyocyanine；Anhydro [5-methyl-1-hydroxyphenazinium hydroxide]；1-Hydroxy-5-methylphenazin-5-ium；5-methylphenazin-5-ium-1-ol
• CAS: 392287-05-7
• 化学式: C₁₃H₁₁N₂O
• 分子量: 211.243
• InChiKey: YNCMLFHHXWETLD-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  16
• 可旋转键数：
  0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.08
• 拓扑面积：
  37
• 氢给体数：
  1
• 氢受体数：
  2

反应信息

• 作为反应物：
  描述：

  pyocyanin 在 乙醇 、 铂 作用下， 生成 5-methyl-5,10-dihydro-phenazin-1-ol
  参考文献：
  名称：

  Wrede; Strack, Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 1929, vol. 181, p. 75

  摘要：

  DOI：
• 作为产物：
  描述：

  1-hydroxy-5-methyl-phenazinium; methyl sulfate 、 alkaline earth salt of/the/ methylsulfuric acid 生成 pyocyanin
  参考文献：
  名称：

  Wrede; Strack, Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 1929, vol. 181, p. 75

  摘要：

  DOI：

文献信息

• METHODS AND SYSTEM FOR INTERFERING WITH VIABILITY OF BACTERIA AND RELATED COMPOUNDS AND COMPOSITIONS
  申请人：NEWMAN Dianne K.

  公开号：US20130022578A1

  公开（公告）日：2013-01-24

  Provided herein are methods and systems for interfering with viability of bacteria and related compounds and compositions.

  本文提供了干扰细菌和相关化合物和组合物生存能力的方法和系统。
• Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from <i>Pseudomonas aeruginosa</i> PAO1
  作者：Dmitri V. Mavrodi、Robert F. Bonsall、Shannon M. Delaney、Marilyn J. Soule、Greg Phillips、Linda S. Thomashow

  DOI：10.1128/jb.183.21.6454-6465.2001

  日期：2001.11

  Two seven-gene phenazine biosynthetic loci were cloned from Pseudomonas aeruginosa PAO1. The operons, designated phzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2 , are homologous to previously studied phenazine biosynthetic operons from Pseudomonas fluorescens and Pseudomonas aureofaciens . Functional studies of phenazine-nonproducing strains of fluorescent pseudomonads indicated that each of the biosynthetic operons from P. aeruginosa is sufficient for production of a single compound, phenazine-1-carboxylic acid (PCA). Subsequent conversion of PCA to pyocyanin is mediated in P. aeruginosa by two novel phenazine-modifying genes, phzM and phzS , which encode putative phenazine-specific methyltransferase and flavin-containing monooxygenase, respectively. Expression of phzS alone in Escherichia coli or in enzymes, pyocyanin-nonproducing P. fluorescens resulted in conversion of PCA to 1-hydroxyphenazine. P. aeruginosa with insertionally inactivated phzM or phzS developed pyocyanin-deficient phenotypes. A third phenazine-modifying gene, phzH , which has a homologue in Pseudomonas chlororaphis , also was identified and was shown to control synthesis of phenazine-1-carboxamide from PCA in P. aeruginosa PAO1. Our results suggest that there is a complex pyocyanin biosynthetic pathway in P. aeruginosa consisting of two core loci responsible for synthesis of PCA and three additional genes encoding unique enzymes involved in the conversion of PCA to pyocyanin, 1-hydroxyphenazine, and phenazine-1-carboxamide.

  摘要 从铜绿假单胞菌中克隆了两个七基因酚嗪生物合成位点 铜绿假单胞菌 PAO1。这些操作子被命名为 phzA1B1C1D1E1F1G1 和 phzA2B2C2D2E2F2G2 与以前研究过的荧光假单胞菌中的酚嗪生物合成操作子同源。 荧光假单胞菌 和 假单胞菌的酚嗪生物合成操作子。 .对不产酚嗪的荧光假单胞菌菌株进行的功能研究表明，铜绿假单胞菌的每个生物合成操作子都与酚嗪生物合成操作子同源。 铜绿假单胞菌 足以生产单一化合物酚嗪-1-羧酸（PCA）。在铜绿假单胞菌中，酚嗪-1-羧酸（PCA）随后转化为芘花青素的过程是由 铜绿微囊藻 由两个新型酚嗪修饰基因介导、 phzM 和 phzS 分别编码推定的酚嗪特异性甲基转移酶和含黄素单氧化酶。表达 phzS 单独在 大肠杆菌 或酶的情况下，不产生焦花青素的 荧光团 导致 PCA 转化为 1-羟基吩嗪。 铜绿菌 具有插入失活的 phzM 或 phzS 会出现缺乏脓青素的表型。第三个酚嗪修饰基因 phzH 在 假单胞菌中的同源基因 铜绿假单胞菌中也发现了第三个酚嗪修饰基因 phzH，该基因在氯葡萄假单胞菌中也有同源物。 铜绿假单胞菌 PAO1。我们的研究结果表明，在铜绿假单胞菌 PAO1 中存在着一个复杂的芘花青素生物合成途径。 铜绿菌 由负责合成 PCA 的两个核心基因位点和编码参与将 PCA 转化为芘花青素、1-羟基吩嗪和吩嗪-1-甲酰胺的独特酶的另外三个基因组成。
• METHOD OF MEASUREMENT OF MATERIAL AND TESTPIECE
  申请人：KABUSHIKI KAISHA KYOTO DAIICHI KAGAKU

  公开号：EP0860695A1

  公开（公告）日：1998-08-26

  A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple analysis is made possible, and easy handling is possible.

  一种测量被分析物的方法，包括以下步骤：根据样品中含有的被分析物的化学反应，使用包括可检测物质的形成反应的反应系统测量可检测物质，其中在包括可检测物质的形成反应的反应系统中导致层状无机化合物的存在、从而实现高灵敏度测量，稳定可检测物质以提高测量精度，提高化学反应速率以实现快速测量，即使在形成不溶性物质的反应体系中也能实现高灵敏度测量。此外，还可以提供一种用于测量被分析物的分析检测件，通过使用反应系统测量可检测物质，该反应系统包括基于样品中所含被分析物的化学反应的可检测物质的形成反应，其中检测件包括至少一个测试部分，该测试部分具有用于检测可检测物质的检测部分，并且至少在测试部分中包含分层无机化合物，从而防止染料或类似物的扩散和洗脱，实现更灵敏、更准确的简单分析，并且易于处理。
• SPECIMEN HAVING CAPABILITY OF SEPARATING SOLID COMPONENT
  申请人：ARKRAY, Inc.

  公开号：EP1271143A1

  公开（公告）日：2003-01-02

  A test strip having the capability of separating blood cells from whole blood, wherein it has a porous reagent layer comprising beads, an inorganic gel and a reagent that causes a detectable reaction with a substance to be detected, and a substrate that supports the reagent layer, wherein the beads are adhered to each other with the inorganic gel, and interstices are formed between the beads to trap a solid. The test strip can thus be used for separating blood cells from a whole blood including blood cells and plasma and detecting a substance to be detected, such as glucose, contained in plasma. The use of the test strip allows the measurement of a substance to be detected even by measurement of a transmitted light, the exhibition of good oxygen permeability, and thus can be used for measuring a substance to be detected with improved accuracy.

  一种能从全血中分离血细胞的试纸，它具有一个多孔试剂层，该试剂层由微珠、无机凝胶和能与待检测物质发生可检测反应的试剂组成，以及一个支撑试剂层的基板，其中微珠与无机凝胶相互粘附，微珠之间形成间隙以吸附固体。因此，该试纸可用于从包括血细胞和血浆在内的全血中分离出血细胞，并检测血浆中的待检测物质，如葡萄糖。使用该试纸甚至可以通过透射光的测量来检测某种物质，显示出良好的透氧性，从而可以更准确地测量某种待检测物质。
• BIOSENSOR AND METHOD FOR PREPARATION THEREOF
  申请人：Arkray, Inc.

  公开号：EP1679508A1

  公开（公告）日：2006-07-12

  The present invention provides a biosensor that can prevent a mediator from being affected by oxygen, thereby allowing an analyte in a sample solution to be measured rapidly and easily with high accuracy. The biosensor can be produced by providing a substrate having electrodes, applying a solvent containing a mediator, a surfactant, a buffer, and a layered inorganic compound to surfaces of the electrodes to form an inorganic gel layer for preventing natural oxidation of the mediator, and forming an enzyme reagent layer containing an oxidoreductase on the inorganic gel layer. In this biosensor, due to the inorganic gel layer, the mediator having been reduced by the reaction between an analyte and the oxidoreductase can be measured electrochemically, without being reoxidized by dissolved oxygen or the like.

  本发明提供了一种生物传感器，它可以防止介质受到氧气的影响，从而快速、简便、高精度地测量样品溶液中的分析物。该生物传感器的制作方法是：提供一个具有电极的基底，在电极表面涂抹含有介质、表面活性剂、缓冲剂和层状无机化合物的溶剂，以形成无机凝胶体层，防止介质自然氧化，并在无机凝胶体层上形成含有氧化还原酶的酶试剂层。在这种生物传感器中，由于无机凝胶体层的存在，被分析物与氧化还原酶反应还原的介质可以通过电化学方法进行测量，而不会被溶解氧或类似物重新氧化。