norepinephrine

• 分子结构分类: 有机化合物 - 苯类化合物 - 酚类
• 英文名称: norepinephrine
• 英文别名: [2-(3,4-dihydroxyphenyl)-2-hydroxyethyl]azanium
• 化学式: C₈H₁₁NO₃*H
• 分子量: 170.188
• InChiKey: SFLSHLFXELFNJZ-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  -1.2
• 重原子数：
  12
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  88.3
• 氢给体数：
  4
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  norepinephrine 生成 norepinephrine quinone
  参考文献：
  名称：

  Electrochemical mediatorless detection of norepinephrine based on MoO3 nanowires

  摘要：

  Achieving direct electron transfer between a biomolecule and modified electrodes is paramount for fabricating advanced biosensor devices. In this report, 1-dimensional (1D) MoO3 nanowires (NWs) were synthesized in a systematic growth evolution study. These MoO3 NWs, with the glassy carbon electrodes (GCEs), were further used as a mediatorless biosensor electrode for the detection of norepinephrine (NE) by cyclic voltammetry and chronoamperometry techniques. The MoO3 NWs/GCE had a magnificent response time of 2 s in the electrochemical detection of NE, with a detection limit of 0.11 mM. This excellent bio-electrochemical performance is attributed to its high catalytic activity and 1D microstructure, providing a path for electron transport and increasing their sensitivity. The MoO3 NWs/GCE also had a promising diffusion constant (D) value of 3.34 x 10(-5)cm(2)/s and a heterogeneous rate constant (k) of 8.03 x 10(-4) cm/s. The modified electrode possessed high stability, reproducibility, and selectivity. (C) 2017 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.electacta.2017.08.187

文献信息

• Mechanistic and Structural Analysis of <i>Drosophila melanogaster</i> Arylalkylamine <i>N</i>-Acetyltransferases
  作者：Daniel R. Dempsey、Kristen A. Jeffries、Jason D. Bond、Anne-Marie Carpenter、Santiago Rodriguez-Ospina、Leonid Breydo、K. Kenneth Caswell、David J. Merkler

  DOI：10.1021/bi5006078

  日期：2014.12.16

  Arylalkylamine N-acetyltransferase (AANAT) catalyzes the penultimate step in the biosynthesis of melatonin and other N-acetylarylalkylamides from the corresponding arylalkylamine and acetyl-CoA. The N-acetylation of arylalkylamines is a critical step in Drosophila melanogaster for the inactivation of the bioactive amines and the sclerotization of the cuticle. Two AANAT variants (AANATA and AANATB) have been identified in D. melanogaster, in which AANATA differs from AANATB by the truncation of 35 amino acids from the N-terminus. We have expressed and purified both D. melanogaster AANAT variants (AANATA and AANATB) in Escherichia coli and used the purified enzymes to demonstrate that this N-terminal truncation does not affect the activity of the enzyme. Subsequent characterization of the kinetic and chemical mechanism of AANATA identified an ordered sequential mechanism, with acetyl-CoA binding first, followed by tyramine. We used a combination of pH-activity profiling and site-directed mutagenesis to study prospective residues believed to function in AANATA catalysis. These data led to an assignment of Glu-47 as the general base in catalysis with an apparent pKa of 7.0. Using the data generated for the kinetic mechanism, structure-function relationships, pH-rate profiles, and site-directed mutagenesis, we propose a chemical mechanism for AANATA.
• Electrochemical mediatorless detection of norepinephrine based on MoO3 nanowires
  作者：Kunda J. Samdani、Dong Woo Joh、Manas K. Rath、Kang Taek Lee

  DOI：10.1016/j.electacta.2017.08.187

  日期：2017.10

  Achieving direct electron transfer between a biomolecule and modified electrodes is paramount for fabricating advanced biosensor devices. In this report, 1-dimensional (1D) MoO3 nanowires (NWs) were synthesized in a systematic growth evolution study. These MoO3 NWs, with the glassy carbon electrodes (GCEs), were further used as a mediatorless biosensor electrode for the detection of norepinephrine (NE) by cyclic voltammetry and chronoamperometry techniques. The MoO3 NWs/GCE had a magnificent response time of 2 s in the electrochemical detection of NE, with a detection limit of 0.11 mM. This excellent bio-electrochemical performance is attributed to its high catalytic activity and 1D microstructure, providing a path for electron transport and increasing their sensitivity. The MoO3 NWs/GCE also had a promising diffusion constant (D) value of 3.34 x 10(-5)cm(2)/s and a heterogeneous rate constant (k) of 8.03 x 10(-4) cm/s. The modified electrode possessed high stability, reproducibility, and selectivity. (C) 2017 Elsevier Ltd. All rights reserved.