3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide | 144077-66-7

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: 3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide
• 英文别名: 3-hydroxy-[2]naphthoic acid biphenyl-2-ylamide；3-Hydroxy-[2]naphthoesaeure-biphenyl-2-ylamid；N-([1,1'-Biphenyl]-2-yl)-3-hydroxynaphthalene-2-carboxamide；3-hydroxy-N-(2-phenylphenyl)naphthalene-2-carboxamide
• CAS: 144077-66-7
• 化学式: C₂₃H₁₇NO₂
• 分子量: 339.393
• InChiKey: PREVIVYOCUYYSG-UHFFFAOYSA-N

物化性质

• 沸点：
  495.4±38.0 °C(Predicted)
• 密度：
  1.275±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  5.8
• 重原子数：
  26
• 可旋转键数：
  3
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  49.3
• 氢给体数：
  2
• 氢受体数：
  2

反应信息

• 作为反应物：
  描述：

  3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide 在 五氯化磷 作用下， 以 1,4-二氧六环 为溶剂， 反应 1.0h， 生成 [3-[(2-苯基苯基)氨基甲酰]萘-2-基]磷酸二氢酯
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022
• 作为产物：
  描述：

  2-氨基联苯 、 2-羟基-3-萘甲酸 在 三氯化磷 作用下， 生成 3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022

文献信息

• Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids
  作者：Satoshi. Fujita、Masayoshi. Momiyama、Yasumitsu. Kondo、Naoto. Kagiyama、Samuel H. Hori、Takeshi. Toru

  DOI：10.1021/ac00080a022

  日期：1994.4.15

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.