5-[(4-Hydroxyphenyl)methylidene]-3-methyl-2-sulfanylidene-1,3-thiazolidin-4-one

• 分子结构分类: 有机化合物 - 苯类化合物 - 酚类
• 英文名称: 5-[(4-Hydroxyphenyl)methylidene]-3-methyl-2-sulfanylidene-1,3-thiazolidin-4-one
• 化学式: C₁₁H₉NO₂S₂
• mdl: MFCD00458828
• 分子量: 251.3
• InChiKey: CCWKDSPKUCORRF-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.6
• 重原子数：
  16
• 可旋转键数：
  1
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.09
• 拓扑面积：
  97.9
• 氢给体数：
  1
• 氢受体数：
  4

文献信息

• SPLIT PHOTOACTIVE YELLOW PROTEIN COMPLEMENTATION SYSTEM AND USES THEREOF
  申请人：Paris Sciences et Lettres - Quartier Latin

  公开号：EP3719007A1

  公开（公告）日：2020-10-07

  The present invention relates to a complementation system comprising two fragments of photoactive yellow protein (PYP), or truncated fragments thereof, and its use with a fluorogenic hydroxybenzylidene rhodanine (HBR) analog for detecting interaction between biological molecules of interest, in particular between proteins of interest. The present invention especially relates to a complementation system comprising a first PYP fragment comprising an amino acid sequence having at least about 70% identity with the amino acid sequence as set forth in SEQ ID NO: 23, or a truncated fragment thereof comprising at least 89 consecutive amino acids from the C-terminal end of said amino acid sequence; and a second PYP fragment comprising an amino acid sequence having at least about 70% identity with the amino acid sequence as set forth in SEQ ID NO: 34, or a truncated fragment thereof comprising at least 8 consecutive amino acids of said amino acid sequence.

  本发明涉及一种由光活性黄蛋白（PYP）的两个片段或其截断片段组成的互补系统，及其与含氟羟基亚苄基罗丹宁（HBR）类似物一起用于检测相关生物分子之间的相互作用，特别是相关蛋白质之间的相互作用。 本发明尤其涉及一种互补系统，该系统包含第一个PYP片段，其氨基酸序列与SEQ ID NO：23 所述氨基酸序列至少具有约 70% 的同一性的氨基酸序列，或其截短片段，该片段包含所述氨基酸序列 C 端起至少 89 个连续氨基酸；以及第二PYP 片段，该片段包含与 SEQ ID NO: 34 所述氨基酸序列至少具有约 70% 的同一性的氨基酸序列，或其截短片段，该片段包含所述氨基酸序列的至少 8 个连续氨基酸。
• Fluorogen activating and shifting tag (FAST)
  申请人：PARIS SCIENCES ET LETTRES -QUARTIER LATIN

  公开号：US10138278B2

  公开（公告）日：2018-11-27

  Disclosed is a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labelling particles, e.g. proteins, or surfaces, which is capable of binding reversibly a fluorogenic chromophore of formula (I), and which is capable of enhancing the brightness of the fluorogenic chromophore upon complexation thereto; and of inducing the spectral shift of the fluorogenic chromophore through the ionization of an auxochromic group thereof.

  本发明公开了一种光活性黄蛋白（PYP）的功能衍生物或其功能片段，用于荧光标记颗粒（如蛋白质）或表面，该衍生物能够可逆地结合式（I）的荧光发色团，与之络合后能够提高荧光发色团的亮度，并通过其辅助色基的电离引起荧光发色团的光谱偏移。
• FLUOROGEN ACTIVATING AND SHIFTING TAG (FAST)
  申请人：Paris Sciences et Lettres - Quartier Latin

  公开号：EP3164411B1

  公开（公告）日：2019-08-28
• [EN] FLUOROGEN ACTIVATING AND SHIFTING TAG (FAST)<br/>[FR] ÉTIQUETTE D'ACTIVATION ET DE DÉCALAGE DU SPECTRE D'UN FLUOROGÈNE (FAST)
  申请人：PARIS SCIENCES LETTRES QUARTIER LATIN

  公开号：WO2016001437A2

  公开（公告）日：2016-01-07

  The present invention relates to a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labelling particles, e.g. proteins, or surfaces, which is capable of binding reversibly a fluorogenic chromophore of formula (I), and which is capable of enhancing the brightness of the said fluorogenic chromophore upon complexation thereto; and of inducing the spectral shift of the said fluorogenic chromophore through the ionization of an auxochromic group thereof.
• [EN] SPLIT PHOTOACTIVE YELLOW PROTEIN COMPLEMENTATION SYSTEM AND USES THEREOF<br/>[FR] SYSTÈME DE COMPLÉMENTATION DE PROTÉINE JAUNE PHOTOACTIVE DIVISÉE ET SES UTILISATIONS
  申请人：PARIS SCIENCES ET LETTRES – QUARTIER LATIN

  公开号：WO2020201538A1

  公开（公告）日：2020-10-08

  The present invention relates to a complementation system comprising two fragments of photoactive yellow protein (PYP), or truncated fragments thereof, and its use with a fluorogenic hydroxybenzylidene rhodanine (HBR) analog for detecting interactions between biological molecules of interest, in particular between proteins of interest. The present invention especially relates to a complementation system comprising a first PYP fragment comprising an amino acid sequence having at least about 70% identity with the amino acid sequence as set forth in SEQ ID NO: 23, or a truncated fragment thereof comprising at least 89 consecutive amino acids from the C -terminal end of said amino acid sequence; and a second PYP fragment comprising an amino acid sequence having at least about 70% identity with the amino acid sequence as set forth in SEQ ID NO: 34, or a truncated fragment thereof comprising at least 8 consecutive amino acids of said amino acid sequence, preferably 8 consecutive amino acids from the N-terminal end of said amino acid sequence.