6-[benzyl(tert-butyl)amino]-1H-pyrimidin-2-one

• 分子结构分类: 有机化合物 - 有机氮化合物
• 英文名称: 6-[benzyl(tert-butyl)amino]-1H-pyrimidin-2-one
• 化学式: C₁₅H₁₉N₃O
• 分子量: 257.33
• InChiKey: DGVDOEKQSXPHKL-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.1
• 重原子数：
  19
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  44.7
• 氢给体数：
  1
• 氢受体数：
  2

文献信息

• Method for preventing high molecular weight products during amplification
  申请人：Roche Diagnostics GmbH

  公开号：EP2722403A1

  公开（公告）日：2014-04-23

  The present invention is directed to improved methods for amplifying and detecting a nucleic acid target that may be present in a biological sample comprising a primer pair for generating an amplicon, wherein at least one primer is modified at the 5' terminus with a polyN sequence, which consists of one type of nucleotide or two different nucleotides, being non-complementary to the target sequence, and a detectable probe specific for said amplicon or a DNA binding dye. The formation of high molecular weight products during amplification is prevented or partly or completely suppressed. The invention further provides use of appropriately modified primers and a kit comprising said primers for preventing or suppressing the formation of high molecular weight products during amplification and detection of the nucleic acid target.

  本发明旨在改进扩增和检测可能存在于生物样品中的核酸靶标的方法，该方法包括用于产生扩增片段的引物对，其中至少一个引物在 5'末端用 polyN 序列修饰，该序列由一种类型的核苷酸或两种不同的核苷酸组成，与靶序列不互补，以及对所述扩增片段特异的可检测探针或 DNA 结合染料。扩增过程中高分子量产物的形成被阻止或部分或完全抑制。本发明进一步提供了经适当修饰的引物和包含所述引物的试剂盒，用于防止或抑制核酸靶标扩增和检测过程中高分子量产物的形成。
• Method for Preventing High Molecular Weight Products During Amplification
  申请人：ROCHE MOLECULAR SYSTEM, INC.

  公开号：US20140113279A1

  公开（公告）日：2014-04-24

  The present invention is directed to improved methods for amplifying and detecting a nucleic acid target that may be present in a biological sample comprising a primer pair for generating an amplicon, wherein at least one primer is modified at the 5′ terminus with a polyN sequence being non-complementary to the target sequence, and a detectable probe specific for said amplicon or a DNA binding dye. The formation of high molecular weight products during amplification is prevented or partly or completely suppressed. The invention further provides reaction mixtures and kits comprising said primers for preventing or suppressing the formation of high molecular weight products during amplification and detection of the nucleic acid target.
• US9447476B2
  申请人：——

  公开号：US9447476B2

  公开（公告）日：2016-09-20