The present invention provides methods for quantifying an amount of plasmalogens in samples with high accuracy in an easy, convenient and inexpensive manner by using a hydrolysis processing to samples and a lipid extraction followed by the measurement using High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) or Mass Spectrometer (MS), a fluorescence plate reader or a plate reader. The present invention also relates to a method for examining a subject by using the above method, a biomarker for disease detection, a method for using the biomarker for the disease detection, as well as a kit for the disease detection.
本发明提供了通过对样品进行
水解处理和脂质提取,然后使用带有蒸发光散射检测器(EL
SD)或质谱仪(MS)的高效
液相色谱法(HPLC)、荧光平板阅读器或平板阅读器进行测量,从而以简单、方便和廉价的方式高精度地定量检测样品中的质粒蛋白含量的方法。本发明还涉及一种利用上述方法对受试者进行检查的方法、一种用于疾病检测的
生物标记物、一种利用
生物标记物进行疾病检测的方法以及一种用于疾病检测的试剂盒。