2-azido-N-[[4-[(1,3-dioxobenzo[de]isoquinolin-4-yl)methyl]phenyl]methyl]acetamide

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 异喹啉及其衍生物
• 英文名称: 2-azido-N-[[4-[(1,3-dioxobenzo[de]isoquinolin-4-yl)methyl]phenyl]methyl]acetamide
• 化学式: C22H17N5O3
• 分子量: 399.4
• InChiKey: GXFXSICRNVLNCZ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.1
• 重原子数：
  30
• 可旋转键数：
  6
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  89.6
• 氢给体数：
  2
• 氢受体数：
  5

文献信息

• NUCLEOTIDES AND APTAMERS CONTAINING BORONIC ACID GROUPS HAVING BIASED BINDING TO GLYCOSYLATED PROTEINS, AND USES THEREOF
  申请人：Georgia State University Research Foundation, Inc.

  公开号：EP2176277A2

  公开（公告）日：2010-04-21
• [EN] NUCLEOTIDES AND APTAMERS CONTAINING BORONIC ACID GROUPS HAVING BIASED BINDING TO GLYCOSYLATED PROTEINS, AND USES THEREOF<br/>[FR] NUCLÉOTIDES ET APTAMÈRES CONTENANT DES GROUPES ACIDE BORONIQUE AYANT UNE LIAISON SOLLICITÉE À DES PROTÉINES GLYCOSYLÉES ET LEURS UTILISATIONS
  申请人：UNIV GEORGIA STATE RES FOUND

  公开号：WO2009012363A2

  公开（公告）日：2009-01-22

  The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein.