5-Bromo-4-chloro-3-indolyl sulfate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机硫酸及其衍生物
• 英文名称: 5-Bromo-4-chloro-3-indolyl sulfate
• 英文别名: (5-bromo-4-chloro-1H-indol-3-yl) hydrogen sulfate
• 化学式: C₈H₅BrClNO₄S
• 分子量: 326.55
• InChiKey: KZKSBQXQOQUOQO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  16
• 可旋转键数：
  2
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  87.8
• 氢给体数：
  2
• 氢受体数：
  4

文献信息

• Enzyme assays
  申请人：Sankyo Company Limited

  公开号：EP0476930A1

  公开（公告）日：1992-03-25

  Accurate enzyme assays suitable for use in immunoassay techniques are now possible for enzymes associated with the dimerisation of indoxyl esters to form the corresponding indigo, the assays utilising the production of hydrogen peroxide from this reaction, which is found to be sufficiently consistent that it can be used to accurately calculate enzyme activity down to levels of 1 in 1000 compared to colorimetric techniques for the same enzymes.

  对于与吲哚基酯二聚形成相应的靛蓝有关的酶，现在可以进行适用于免疫测定技术的精确酶测定，这种测定利用了从这一反应中产生的过氧化氢。
• Genetically purified gellan gum
  申请人：CP Kelco, U.S., Inc.

  公开号：EP2325323A1

  公开（公告）日：2011-05-25

  Mutational inactivation of proteins involved in para-cresol production in certain milk products results in improved taste and odour. The undesirable para-cresol forms over time as a result of enzymes produced by the bacterium that produces gellan gum. Since the gellan is typically used in a relatively unpurified form, the enzymes are added to the milk along with the gellan. Inactivation of the enzymes is a genetic means of eliminating the enzymes without requiring any additional purification or processing.

  对某些奶制品中参与对甲酚生成的蛋白质进行突变失活，可改善口感和气味。由于生产结冷胶的细菌会产生酶，随着时间的推移会形成不受欢迎的对甲酚。由于结冷胶通常以相对未经纯化的形式使用，因此酶会与结冷胶一起添加到牛奶中。酶的失活是消除酶的一种遗传方法，不需要任何额外的纯化或加工。
• Biological indicator
  申请人：American Sterilizer Company

  公开号：US10011843B2

  公开（公告）日：2018-07-03

  This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

  本发明涉及一种生物指示剂，其成分包括：由形成孢子的细菌组成的宿主生物；产生指示剂酶的报告基因；调控基因；以及将报告基因和调控基因插入宿主生物的载体；宿主生物的基因组中含有转座遗传因子，用于在调控基因中插入插入序列；插入序列包括转座酶、一对末端倒置重复序列和至少一个用于表达转座酶的开放阅读框。载体可被宿主生物吸收。插入序列可插入调控基因中。宿主生物可进行孢子繁殖以形成生物指示剂。本发明公开了一种使用生物指示剂的工艺和装置。
• Method for detecting Escherichia coli in a sample
  申请人：3M INNOVATIVE PROPERTIES COMPANY

  公开号：US10023898B2

  公开（公告）日：2018-07-17

  A method of detecting Listeria monocytogenes. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of Listeria microorganisms. When a Listeria microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect Listeria monocytogenes.

  一种检测李斯特菌的方法。该方法包括提供一个带有选择性培养基的培养装置和一个包含第一指示系统的检测物品。选择性培养基有利于李斯特菌微生物的生长。当在与培养基接触的样品中检测到李斯特菌微生物时，检测物品与培养基接触以检测单增李斯特菌。
• Method for detecting a Shigella or Cronobacter microorganism
  申请人：3M INNOVATIVE PROPERTIES COMPANY

  公开号：US10619181B2

  公开（公告）日：2020-04-14

  A method of detecting a Shigella or Cronobacter microorganism. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system.

  一种检测志贺氏菌或克罗诺斯菌微生物的方法。该方法包括提供一个带有选择性培养基的培养装置和一个包含第一指示系统的检测物品。