The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA).
本发明涉及一种预涂层基底,如载玻片,可用于固定样品。本发明进一步提供了制备这种预涂层基底的方法和分析固定在这种预涂层基底上的
生物样本的方法。在基底上涂覆特选的聚阳离子聚合物涂层材料,使涂覆基底具有更高的稳定性和更长的保存期限。优选的聚合物涂层材料包括烯丙基或
乙烯基聚合物,这些聚合物上有阳离子基团,并且肽单体连接的比例不超过一小部分,特别是聚
二烯丙基二甲基
铵(P
DDA)。