r-(+)-alpha-(3,4-Dihydroxyphenyl)-lactic acid

• 分子结构分类: 有机化合物 - 苯类化合物 - 酚类
• 英文名称: r-(+)-alpha-(3,4-Dihydroxyphenyl)-lactic acid
• 英文别名: (2R)-2-(3,4-dihydroxyphenyl)-2-hydroxypropanoic acid
• 化学式: C₉H₁₀O₅
• 分子量: 198.17
• InChiKey: NDGMYGNFKSMYBB-SECBINFHSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.3
• 重原子数：
  14
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  98
• 氢给体数：
  4
• 氢受体数：
  5

文献信息

• Engineering strain and application thereof in production of Danshensu
  申请人：Jiangnan University

  公开号：US10870870B2

  公开（公告）日：2020-12-22

  The present disclosure discloses an engineering strain and application thereof in joint production of Danshensu and alanine, and belongs to the technical field of bioengineering. The present disclosure constructs a three-enzyme co-expression genetic engineering strain, and realizes joint production of Danshensu and alanine. Further, the transport of a substrate is promoted and decomposition of products is reduced by knocking out or enhancing expression of related genes on E. coli genome. The genetic engineering strain provided by the present disclosure can produce optically pure D-danshensu and L-danshensu, and jointly produce pyruvic acid. The production process is simple, raw materials are easily available, impurities are fewer, and a good industrial application prospect is achieved.

  本发明公开了一种工程菌株及其在丹参素和丙氨酸联合生产中的应用，属于生物工程技术领域。本发明构建了一种三酶共表达基因工程菌株，实现了丹参素和丙氨酸的联合生产。此外，通过敲除或增强大肠杆菌基因组中相关基因的表达，促进底物的转运，减少产物的分解。本公开提供的基因工程菌株可以生产光学纯度的 D-丹参素和 L-丹参素，并联合生产丙酮酸。生产工艺简单，原料易得，杂质少，具有良好的工业应用前景。
• Recombinant E. coli and method of producing Danshensu by using same
  申请人：Jiangnan University

  公开号：US10829790B2

  公开（公告）日：2020-11-10

  The present disclosure discloses a production method of Danshensu, belonging to the technical field of bioengineering. The present disclosure constructs a novel genetic engineering strain co-expressed by three enzymes, which can be applied to the production of optically pure 3-(3,4-dihydroxyphenyl)-2-hydroxypropionic acid. All of the (D/L)-α-hydroxycarboxylic acid dehydrogenase selected by the present disclosure have the characteristics of poor substrate specificity and strong optical specificity, and can produce optically pure D-danshensu and L-danshensu. Further, the production efficiency of the recombinant strain is improved by knocking out or enhancing the expression of a related gene on the E. coli genome to promote substrate transport and reduce product decomposition. The method for producing Danshensu and α-ketoglutaric acid by using the transformation of the recombinant strain according to the present disclosure is simple, has easily available raw materials, few impurities, and has good industrial application prospects.

  本发明公开了一种丹参素的生产方法，属于生物工程技术领域。本公开构建了一种由三种酶共同表达的新型基因工程菌株，可用于生产光学纯的 3-(3,4-二羟基苯基)-2-羟基丙酸。本发明所选择的（D/L）-α-羟基羧酸脱氢酶均具有底物专一性差、光学专一性强的特点，可生产光学纯的D-丹参素和L-丹参素。此外，通过敲除或增强大肠杆菌基因组中相关基因的表达，促进底物转运，减少产物分解，从而提高重组菌株的生产效率。根据本公开内容，利用重组菌株转化生产丹参素和α-酮戊二酸的方法简单，原料易得，杂质少，具有良好的工业应用前景。