azido biotin

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 生物素及其衍生物
• 英文名称: azido biotin
• 英文别名: azido 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate
• 化学式: C₁₀H₁₅N₅O₃S
• 分子量: 285.327
• InChiKey: NZFLBRLTKNMVRJ-ZKWXMUAHSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.9
• 重原子数：
  19
• 可旋转键数：
  7
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.8
• 拓扑面积：
  107
• 氢给体数：
  2
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  氨氧基-一聚乙二醇-叠氮 、 D-生物素 以 N,N-二甲基甲酰胺 为溶剂， 以88%的产率得到azido biotin
  参考文献：
  名称：

  REAGENTS AND METHODS FOR SIRTUIN CAPTURE

  摘要：

  这项发明提供了一种制备sirtuin复合物的方法。该发明还提供了一种检测样品中sirtuin的方法，包括使用上述sirtuin复合物。

  公开号：

  US20130065248A1

文献信息

• [EN] LN(III) AND GA(III) METALLACROWN COMPLEXES<br/>[FR] COMPLEXES DE MÉTALLACOURONNES CONTENANT LN (III) ET GA (III)
  申请人：UNIV MICHIGAN REGENTS

  公开号：WO2016166380A1

  公开（公告）日：2016-10-20

  The present invention concerns heterometallic metallacrown compounds incorporating Ga(lll) and Ln(lll) cations, with a templating ligand such as salicylhydroxamic acid or derivatives thereof, wherein said metallacrown contains at least one repeating [-Ga-N-O-] sub-unit where the N-0 derives from the templating ligand.

  本发明涉及含有Ga(lll)和Ln(lll)阳离子的异金属金属冠化合物，其中包含模板配体，例如水杨酰羟胺酸或其衍生物，该金属冠至少包含一个重复的[-Ga-N-O-]亚基，其中N-O来源于模板配体。
• Methods
  申请人：Nguyen Duy P.

  公开号：US20120077948A1

  公开（公告）日：2012-03-29

  The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

  本发明涉及一种制备具有正交功能基团的多肽的方法，所述正交功能基团由脂肪族氨基酸或氨基酸衍生物组成，该方法包括提供宿主细胞；提供编码所需多肽的核酸；提供与所述宿主细胞正交的tRNA-tRNA合成酶对；添加包含所需正交功能基团的氨基酸或氨基酸衍生物，其中所述氨基酸或氨基酸衍生物是正交tRNA合成酶的底物，其中所述氨基酸或氨基酸衍生物具有脂肪族碳骨架；孵育以允许通过正交tRNA-tRNA合成酶对将所述氨基酸或氨基酸衍生物并入所需多肽中。本发明还涉及某些氨基酸以及包含它们的多肽。
• CLEAVABLE PROBES FOR ISOTOPE TARGETED GLYCOPROTEOMICS AND METHODS OF USING THE SAME
  申请人：The Regents of the University of California

  公开号：US20160187350A1

  公开（公告）日：2016-06-30

  Methods for producing isotopically-labelled peptides are provided. Aspects of the method include: contacting a sample including a metabolically tagged protein with a cleavable probe to produce a probe-protein conjugate; separating the probe-protein conjugate from the sample; digesting the probe-protein conjugate to produce a probe-peptide conjugate; and cleaving a cleavable linker to release an isotopically labelled peptide. The method may further include: identifying a predetermined isotopic pattern in a mass spectrum; determining an amino acid sequence of the isotopically labelled peptide; and identifying the site of protein glycosylation based on the determined amino acid sequence. Also provided are cleavable probes for practicing the subject methods, described by the Formula: A-L-(M-Z) where A is an affinity tag, L is a cleavable linker, M is an isotopic label and Z is a chemoselective tag capable of cross-linking a metabolically tagged protein. Compositions and kits for practicing the subject methods are also provided.

  提供生产同位素标记肽的方法。该方法的步骤包括：将含有代谢标记蛋白质的样品与可裂解的探针接触以产生探针-蛋白质结合物；将探针-蛋白质结合物与样品分离；消化探针-蛋白质结合物以产生探针-肽结合物；裂解可裂解的连接剂以释放同位素标记的肽。该方法还可以包括：在质谱中识别预定的同位素模式；确定同位素标记的肽的氨基酸序列；并根据确定的氨基酸序列确定蛋白质糖基化的位置。还提供了可裂解的探针，用于实施该方法，其由公式A-L-(M-Z)描述，其中A是亲和标记，L是可裂解连接剂，M是同位素标记，Z是具有交联代谢标记蛋白质的化学选择标记。还提供了用于实施该方法的组合物和工具包。
• Tailored glycoproteomic methods for the sequencing, mapping and identification of cellular glycoproteins
  申请人：Wong Chi-Huey

  公开号：US20080299595A1

  公开（公告）日：2008-12-04

  The present disclosure relates to tailored glycoproteomic methods, and more particularly to methods for the sequencing, mapping and identification of cellular glycoproteins using saccharide-selective bioorthogonal probes. A method is disclosed for saccharide-selective glycoprotein identification (ID) and glycan mapping (GIDmap) that generates glycoproteins tailored with bioorthogonally tagged alkynyl saccharides that can be selectively isolated, allowing for glycoprotein ID and glycan mapping via mass spectromic proteomics, including liquid chromatography-tandmen mass spectroscopy (LC-MS 2 ). LC-MS 2 may be used to identify cellular glycans, and more specifically cancer-related glycoproteins.

  本公开涉及定制的糖蛋白质组学方法，更具体地涉及使用糖基选择性生物正交探针对细胞糖蛋白质进行测序、映射和鉴定的方法。公开了一种糖基选择性糖蛋白质鉴定(ID)和糖基映射(GIDmap)的方法，该方法生成了定制的生物正交标记的炔基糖，可以被选择性地分离，从而通过质谱蛋白质组学，包括液相色谱-串联质谱(LC-MS2)进行糖蛋白质鉴定和糖基映射。LC-MS2可用于鉴定细胞糖基，更具体地是癌症相关的糖蛋白质。
• Cleavable probes for isotope targeted glycoproteomics and methods of using the same
  申请人：The Regents of the University of California

  公开号：US10114026B2

  公开（公告）日：2018-10-30

  Methods for producing isotopically-labelled peptides are provided. Aspects of the method include: contacting a sample including a metabolically tagged protein with a cleavable probe to produce a probe-protein conjugate; separating the probe-protein conjugate from the sample; digesting the probe-protein conjugate to produce a probe-peptide conjugate; and cleaving a cleavable linker to release an isotopically labelled peptide. The method may further include: identifying a predetermined isotopic pattern in a mass spectrum; determining an amino acid sequence of the isotopically labelled peptide; and identifying the site of protein glycosylation based on the determined amino acid sequence. Also provided are cleavable probes for practicing the subject methods, described by the Formula: A-L-(M-Z) where A is an affinity tag, L is a cleavable linker, M is an isotopic label and Z is a chemoselective tag capable of cross-linking a metabolically tagged protein. Compositions and kits for practicing the subject methods are also provided.

  本文提供了生产同位素标记肽的方法。该方法的各个方面包括：将包括代谢标记蛋白质的样品与可裂解探针接触，生成探针-蛋白质共轭物；将探针-蛋白质共轭物与样品分离；消化探针-蛋白质共轭物，生成探针-肽共轭物；裂解可裂解连接体，释放同位素标记的肽。该方法可进一步包括：确定质谱中的预定同位素模式；确定同位素标记肽的氨基酸序列；以及根据确定的氨基酸序列确定蛋白质糖基化的位点。此外，还提供了用于实施上述方法的可裂解探针，其描述式如下：A-L-(M-Z)，其中 A 是亲和性标记，L 是可裂解连接体，M 是同位素标记，Z 是化学选择性标记，能够交联代谢标记蛋白质。此外，还提供了用于实施上述方法的组合物和试剂盒。