3-(3-amino-6-iminoxanthen-9-yl)-N-[2-[2-(6-chlorohexoxy)ethoxy]ethyl]-4-formylbenzamide

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 3-(3-amino-6-iminoxanthen-9-yl)-N-[2-[2-(6-chlorohexoxy)ethoxy]ethyl]-4-formylbenzamide
• 化学式: C₃₁H₃₄ClN₃O₅
• 分子量: 564.1
• InChiKey: KBAWSEKFAXWAAB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.1
• 重原子数：
  40
• 可旋转键数：
  15
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  124
• 氢给体数：
  3
• 氢受体数：
  7

文献信息

• Covalent tethering of functional groups to proteins and substrates therefor
  申请人：Promega Corporation

  公开号：EP2395078A2

  公开（公告）日：2011-12-14

  A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

  提供了一种可与相关蛋白质融合的突变水解酶。突变型水解酶能够与相应的非突变型（野生型）水解酶的底物形成键合，这种键合比野生型水解酶与底物之间形成的键合更稳定，并且相对于野生型水解酶至少有两个氨基酸取代。本发明还提供了包含一个或多个官能团的水解酶底物，以及使用本发明的突变水解酶和底物的方法。还提供了一种能与底物形成稳定结合的融合蛋白以及表达该融合蛋白的细胞。
• COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS
  申请人：PROMEGA CORPORATION

  公开号：EP1594962B1

  公开（公告）日：2014-08-27
• COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR
  申请人：PROMEGA CORPORATION

  公开号：EP1782071B1

  公开（公告）日：2014-10-08
• Covalent tethering of functional groups to proteins
  申请人：Promega Corporation

  公开号：EP2395358B1

  公开（公告）日：2017-03-29