2-[5-[3-(5-Carboxypentyl)-1,1-dimethyl-6,8-disulfobenzo[e]indol-3-ium-2-yl]penta-2,4-dienylidene]-3-ethyl-1,1-dimethyl-8-(trioxidanylsulfanyl)benzo[e]indole-6-sulfonate

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: 2-[5-[3-(5-Carboxypentyl)-1,1-dimethyl-6,8-disulfobenzo[e]indol-3-ium-2-yl]penta-2,4-dienylidene]-3-ethyl-1,1-dimethyl-8-(trioxidanylsulfanyl)benzo[e]indole-6-sulfonate
• 英文别名: 2-[5-[3-(5-carboxypentyl)-1,1-dimethyl-6,8-disulfobenzo[e]indol-3-ium-2-yl]penta-2,4-dienylidene]-3-ethyl-1,1-dimethyl-8-(trioxidanylsulfanyl)benzo[e]indole-6-sulfonate
• 化学式: C41H44N2O14S4
• 分子量: 917.1
• InChiKey: ODBDVLYXZKLHKG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.5
• 重原子数：
  61
• 可旋转键数：
  15
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.32
• 拓扑面积：
  299
• 氢给体数：
  4
• 氢受体数：
  16

文献信息

• METHOD FOR DETECTING SINGLE BASE SUBSTITUTION USING ION EXCHANGE CHROMATOGRAPHY
  申请人：Sekisui Medical Co., Ltd.

  公开号：EP3690039A1

  公开（公告）日：2020-08-05

  An object of the present invention is to provide a method for accurately and quantitatively discriminating and detecting a wide variety of gene mutations, or particularly, single base substitutions or point mutations. In an ASP for analyzing gene mutations, or particularly, single base substitutions or point mutations, when a non-nucleotide component is added to the 5' end of at least one of the ASP and a primer paired therewith before amplification by PCR and amplification products thereof are separated by ion-exchange chromatography, even the amplification products having the same length can be separated and detected.

  本发明的目的是提供一种方法，用于准确和定量地鉴别和检测各种基因突变，特别是单碱基替换或点突变。在用于分析基因突变，特别是单碱基置换或点突变的 ASP 中，如果在 PCR 扩增前在至少一种 ASP 的 5'端和与之配对的引物中加入非核苷酸成分，并用离子交换色谱法分离其扩增产物，即使是具有相同长度的扩增产物也能被分离和检测出来。