The invention concerns the use of a fluorescent protein selected in particular among the autofluorescent proteins for detecting the non-covalent interaction between a target protein marked by the fluorescent protein and one of its ligands marked by a marker consisting of: either a molecule capable of absorbing the light emitted by the fluorescent protein, or a fluorescent substance, said detection taking place by fluorescence energy transfer: between the fluorescent protein and said fluorescent substance, the fluorescent substance being such that it is excitable at the fluorescent protein emitting wavelength, or it emits at the fluorescent protein emitting wavelength; between the fluorescent protein and said molecule capable of absorbing the light emitted by the fluorescent protein.
本发明涉及使用一种荧光蛋白,尤其是自发荧光蛋白,用于检测荧光蛋白标记的目标蛋白与其
配体之间的非共价相互作用,
配体标记包括:能够吸收荧光蛋白发出的光的分子或荧光物质,所述检测通过荧光能量转移进行:荧光蛋白和所述荧光物质之间,荧光物质在荧光蛋白发光波长下可激发,或在荧光蛋白发光波长下发光;荧光蛋白和所述能够吸收荧光蛋白发光的分子之间。