Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer, where the sieving polymer is more hydrophilic than the coating polymer and is present in greater amount. Of particular interest are uncrosslinked acrylamide polymer mixtures for coating plastic channels and providing sieving for performing DNA separations in microfluidic devices. Polyacrylamide or N,N-dimethyl acrylamide is used with a N,N-dialkyl acrylamide copolymer, either separately or together for sieving and coating, serving as the medium in capillary electrophoresis DNA separations.
本发明提供了使用一种组合物进行毛细管电泳的组合物和方法,该组合物在
水性缓冲介质中由包覆聚合物和筛分聚合物组合而成,其中筛分聚合物比包覆聚合物更亲
水,且含量更高。非交
联丙烯酰胺聚合物混合物用于涂覆塑料通道并提供筛分,以便在微流体装置中进行 DNA 分离,这种混合物尤其值得关注。聚
丙烯酰胺或 N,N-二甲基
丙烯酰胺与 N,N-二烷基
丙烯酰胺共聚物可单独或一起用于筛分和涂层,作为毛细管电泳 DNA 分离的介质。